LOCUS       sequence-109564-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. mCherry reporter under the 
            control of a tet-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: Tet-inducible mCherry reporter
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..720)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(288..307)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     557..575
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      717..726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(811..835)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ataagatctt tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg
       61 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt
      121 gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
      181 agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc
      241 gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
      301 tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
      361 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
      421 gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg
      481 gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa
      541 gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
      601 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
      661 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat
      721 ggtggcgaat tcggtaccgg ggccgcggag gctggatcgg tcccggtgtc ttctatggag
      781 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct
      841 tatatagggc tagctcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat
      901 atagacgttg tcgctgatgg cgtt
//
