LOCUS       sequence-109568-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 245 to 279)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-2
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      149..176
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        245..279
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
       61 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
      121 gaacctaggc cttactgcag atcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      181 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttatttagga
      241 tccattgacg gctagctcag tccaaggtac agtgctagct tctagaataa ccatatacca
      301 tttcagcata gatcatatgg tcgacaaggg cgaagaactc tttaccggcg tcgtcccgat
      361 cctggtagag cttgacggcg atgtcaacgg tcataagttt agcgttagcg gcgaaggcga
      421 gggcgacgcg acgtacggta aattaacctt aaaattcatc tgtacaaccg gtaaattacc
      481 ggtaccgtgg cccacgttag ttaccaccct tacctggggc gtccagtgct tcgcgcgcta
      541 tccagaccac atgaagcaac acgatttctt caaatcggct atgcccgaag gctatgtaca
      601 ggagcgcacc atcttcttca aggacgacgg taattataaa acccgcgcag aagttaagtt
      661 cgagggcgat acgctcgtta atcgcatcga actcaaaggt atcgacttca aagaggacgg
      721 caacatttta ggtcacaagt tagagtataa cgcgatttcc gataacgtct acattaccgc
      781 cgat
//
