LOCUS       sequence-109569-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 244 to 278)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-9
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      148..175
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        244..278
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
ORIGIN
        1 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
       61 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
      121 aacctaggcc ttactgcaga tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      181 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tatttaggat
      241 ccattgacgg ctagctcagt ccaaggtaca gtgctagctt ctagacgaaa tagatctaaa
      301 tagggaggca ggaaaaatgg tcgacaaggg cgaagaactc tttaccggcg tcgtcccgat
      361 cctggtagag cttgacggcg atgtcaacgg tcataagttt agcgttagcg gcgaaggcga
      421 gggcgacgcg acgtacggta aattaacctt aaaattcatc tgtacaaccg gtaaattacc
      481 ggtaccgtgg cccacgttag ttaccaccct tacctggggc gtccagtgct tcgcgcgcta
      541 tccagaccac atgaagcaac acgatttctt caaatcggct atgcccgaag gctatgtaca
      601 ggagcgcacc atcttcttca aggacgacgg taattataaa acccgcgcag aagttaagtt
      661 cgagggcgat acgctcgtta atcgcatcga actcaaaggt atcgacttca aagaggacgg
      721 caacatttta ggtcacaagt tagagtataa cgcgatttcc gataacgtct acattaccgc
      781 cgat
//
