LOCUS       sequence-109577-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 245 to 279)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-10
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      149..176
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        245..279
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             305..313
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
       61 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
      121 gaacctaggc cttactgcag atcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      181 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttatttagga
      241 tccattgacg gctagctcag tccaaggtac agtgctagct tctagaccct caagcaaggg
      301 gatataagga ggtaccaata tggtcgacaa gggcgaagaa ctctttaccg gcgtcgtccc
      361 gatcctggta gagcttgacg gcgatgtcaa cggtcataag tttagcgtta gcggcgaagg
      421 cgagggcgac gcgacgtacg gtaaattaac cttaaaattc atctgtacaa ccggtaaatt
      481 accggtaccg tggcccacgt tagttaccac ccttacctgg ggcgtccagt gcttcgcgcg
      541 ctatccagac cacatgaagc aacacgattt cttcaaatcg gctatgcccg aaggctatgt
      601 acaggagcgc accatcttct tcaaggacga cggtaattat aaaacccgcg cagaagttaa
      661 gttcgagggc gatacgctcg ttaatcgcat cgaactcaaa ggtatcgact tcaaagagga
      721 cggcaacatt ttaggtcaca agttagagta taacgcgatt tccgataacg tctacattac
      781 cgccgat
//
