LOCUS       sequence-109581-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS, Ho1, and PcyA constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR43.6r
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             163..174
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tgtggatgaa gccaatgacg cctttgccat gaacatgaaa atgttcaacg aacttgaagg
       61 caacctgatc aaggcgatcg gcattatggt gttcaacagc ctcacccgtc gccgcagtca
      121 aggcagcacc gaagttggcc tcgccacctc cgaaggctag ttaaagagga gaaaggatcc
      181 atggccgtca ctgatttaag tttgaccaat tcttccctga tgcccacgtt gaacccgatg
      241 attcaacagt tggccctggc gatcgccgct agttggcaaa gtttacccct caagccctat
      301 caattgccgg aggatttggg ctacgtagaa ggccgcctgg aaggggaaaa gttagtgatt
      361 gaaaatcggt gctaccaaac gccccagttt cgcaaaatgc atttggagtt ggccaaggtg
      421 ggcaaagggt tggatattct ccactgtgta atgtttcctg agcctttata cggtctacct
      481 ttgtttggct gtgacattgt ggccggcccc ggtggagtaa gtgcggctat tgcggatcta
      541 tcccccaccc aaagcgatcg ccaattgccc gcagcgtacc aaaaatcatt ggcagagcta
      601 ggccagccag aatttgagca acaacgggaa ttgcccccct ggggagaaat attttctgaa
      661 tattgtttat tcatccgtcc cagcaatgtc actgaagaag aaagatttgt acaaagggta
      721 gtggactttt tgcaaattca ttgtcaccaa tccatcgttg ccgaaccctt gtctgaagct
      781 caaactttgg agcaccgtca ggggcaaatt cattactgcc aacaacaaca gaaaaatgat
      841 aaaacccgtc gggtactgga aaaagctttt ggggaagctt gggcggaacg gtatatgagc
      901 caagtcttat ttgatgttat ccaataatct agaccaggca tcaaataaaa cgaaa
//
