LOCUS       sequence-109589-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Mammalian expression of 
            sgRNA1 to target pNeurog2-Luciferase reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: sgRNA1_pNeurog2-Luciferase reporter
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     43..63
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        45..63
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_RNA        complement(97..172)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(194..442)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(422..442)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(456..475)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(457..475)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(485..502)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(485..501)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(494..516)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(730..749)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
       61 agggaacaaa agctggagct ccaccgcgga aaaaaagcac cgactcggtg ccactttttc
      121 aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa accatcgtat
      181 tatcatcatt cacggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat
      241 actttcaagt tacgataagc atatgatagt ccattttaaa acataatttt aaaactgcaa
      301 actacccaag aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta
      361 cagtcaaatt aattccaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag
      421 gaatcatggg aaataggccc tcggtaccca attcgcccta tagtgagtcg tattacgcgc
      481 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      541 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      601 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
      661 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      721 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      781 gtcaagctct aaatcgggg
//
