LOCUS       sequence-109599-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CTGA-R13-AKSIRV RNAP mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Meyer AJ, Ellefson JW, Ellington AD
  TITLE     Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase 
            Variants for in Vivo or in Vitro Synthetic Circuitry.
  JOURNAL   ACS Synth Biol. 2014 Oct 7.
  PUBMED    25279711
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Oct 7."
COMMENT     Sequence Label: pQE-WT-CTGA-R13-AKSIRV
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggatctatca acaggagtcc aagctcagct aattaagctt ttacgcgaac gcgaagtccg
       61 actctaagat gtcacggagg ttcaagttac ctttagccgg aagtgctggc attttgtcca
      121 attgagactc gtgcaactgg tcagcgaact ggtcgtagaa atcagccagt acatcacaag
      181 actcatatgt gtcaaccata gtttcgcgca ctgctttgaa caggttcgca gcgtcagccg
      241 gaatggtacc gaaggagtcg tgaatcagtg caaaagattc gattccgtac ttctcgtgtg
      301 cccacactac agtcttacga aggtggctac cgtcttggct gtgtacaaag ttaggagcga
      361 taccagacac ctgtttgcgt gcatcaatct cgctatcttt gttggtgtta atggtaggct
      421 ttaaggtgaa ctgaccgagg aacattatag agatgcgcgt tttaataggc ttcttgtatt
      481 cctgccacac agggaaacca tcaggagtta cccaatgcgc agcgcaacgc ttgcgaagaa
      541 tctctccagt cttcttatct ttgacctcag cagccagcag cttagcagca gacttaagcc
      601 agttcattgc ttcaaccgca gctaccaccg tcacgctcac agattcccaa atcagcttag
      661 ccatgtatcc agcagcctga ttcggctgag tgaacatcag acccttgccg gaatcaatag
      721 ctggctgaat ggtatcttcc agcacttgtt gacggaagcc gaactctttg gacccgtaag
      781 ccagcgtcat gactgaacgc ttagtcacac tgcgagtaac accgtaagcc agccattgac
      841 cagccagtgc cttagtgcc
//
