LOCUS       sequence-109600-         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the Azure (blue) spectral variant of eGFP in bacteria 
            and in mammalian cells. Could be used as a reporter for quantifying 
            gene targeting and recombineering in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Valledor M, Hu Q, Schiller P, Myers RS
  TITLE     Fluorescent protein engineering by in vivo site-directed 
            mutagenesis.
  JOURNAL   IUBMB Life. 2012 Aug;64(8):684-9. doi: 10.1002/iub.1041. Epub 2012 
            May 28.
  PUBMED    22639380
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/iub.1041"; 
            journalName: "IUBMB Life"; date: "2012-08"; volume: "64"; issue: 
            "8"; pages: "684-9"
COMMENT     Sequence Label: pDual-eGFP(H66)
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(132..153)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     481..500
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccaggcacaa gcagcattgt tagctgctgt attgctactt gtgattgctc catgtttttc
       61 taggtctcga ggtcggatct gagtccggac ttgtacagct cgtccatgcc gagagtgatc
      121 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      181 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      241 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      301 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
      361 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      421 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      481 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      541 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
      601 acgccgtggg tcagggtggt cacgagggtg ggccaggg
//
