LOCUS       sequence-109608-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, N1 Cloning Vector, Excitation:587, 
            Emission:610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CenpA-N-22
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    58..110
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      527..536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(563..766)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(592..612)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtggc gaccggtgga
       61 tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgagctcgag gccgagtccc
      121 tcctcaaggc cccggatcct ccgggccagt tgcacatcct ttgggaagag agtaactcgg
      181 cctgcatgta aggtgaggag ataggcgtcc tcaaagagat gaactagaaa tgcttctgct
      241 gcctcttgta gggccaatag ggcctgggct tgccaattga agtccacacc acgagtgaat
      301 ttaacacata tttctcttgc caggcggctg aagggcagct tccttatcaa gaggtgtgtg
      361 ctcttctgaa gctttcggat ctcctttagc caaccttgtc tccgccgact gtgttgatgg
      421 gaggaagcgc ctaaggaggg gccccgccgg gaggggccgg gggtcggggt cgggctcggg
      481 ctgcgcctcc tcggggcctc gggctttcgg ctccggcggc gcgggcccat ggtggctagc
      541 ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac
      601 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
      661 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
      721 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
      781 tgactaa
//
