LOCUS       sequence-109616-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Mito-7
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..104
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             138..818
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagca tgtccgtcct gacgccgctg ctgctgcggg
       61 gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc caagatccat tcgttggggg
      121 atccaccggt cgccaccatg agtgcgatta agccagacat gaagatcaaa ctccgtatgg
      181 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg
      241 agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
      301 atatcctgac cactgcattc cattacggca acagggtatt cgccaaatat ccagacaaca
      361 tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt
      421 tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg gacactttct
      481 ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt atgcagaaga
      541 agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg
      601 gtgatattca tatggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa
      661 ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt gtggaccact
      721 gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg
      781 ttgctcattc tggattgcct gacaatgcca gacgataagc ggccgcgact ctaga
//
