LOCUS       sequence-109622-        3969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3969)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Integrin-Beta1-N-18
FEATURES             Location/Qualifiers
     source          1..3969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2528..3205
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             3254..3931
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
       61 agtcgacggt accgccacca tgaatttaca accaattttc tggattggac tgatcagttc
      121 agtttgctgt gtgtttgctc aaacagatga aaatagatgt ttaaaagcaa atgccaaatc
      181 atgtggagaa tgtatacaag cagggccaaa ttgtgggtgg tgcacaaatt caacattttt
      241 acaggaagga atgcctactt ctgcacgatg tgatgattta gaagccttaa aaaagaaggg
      301 ttgccctcca gatgacatag aaaatcccag aggctccaaa gatataaaga aaaataaaaa
      361 tgtaaccaac cgtagcaaag gaacagcaga gaagctcaag ccagaggata ttactcagat
      421 ccaaccacag cagttggttt tgcgattaag atcaggggag ccacagacat ttacattaaa
      481 attcaagaga gctgaagact atcccattga cctctactac cttatggacc tgtcttactc
      541 aatgaaagac gatttggaga atgtaaaaag tcttggaaca gatctgatga atgaaatgag
      601 gaggattact tcggacttca gaattggatt tggctcattt gtggaaaaga ctgtgatgcc
      661 ttacattagc acaacaccag ctaagctcag gaacccttgc acaagtgaac agaactgcac
      721 cagcccattt agctacaaaa atgtgctcag tcttactaat aaaggagaag tatttaatga
      781 acttgttgga aaacagcgca tatctggaaa tttggattct ccagaaggtg gtttcgatgc
      841 catcatgcaa gttgcagttt gtggatcact gattggctgg aggaatgtta cacggctgct
      901 ggtgttttcc acagatgccg ggtttcactt tgctggagat gggaaacttg gtggcattgt
      961 tttaccaaat gatggacaat gtcacctgga aaataatatg tacacaatga gccattatta
     1021 tgattatcct tctattgctc accttgtcca gaaactgagt gaaaataata ttcagacaat
     1081 ttttgcagtt actgaagaat ttcagcctgt ttacaaggag ctgaaaaact tgatccctaa
     1141 gtcagcagta ggaacattat ctgcaaattc tagcaatgta attcagttga tcattgatgc
     1201 atacaattcc ctttcctcag aagtcatttt ggaaaacggc aaattgtcag aaggagtaac
     1261 aataagttac aaatcttact gcaagaacgg ggtgaatgga acaggggaaa atggaagaaa
     1321 atgttccaat atttccattg gagatgaggt tcaatttgaa attagcataa cttcaaataa
     1381 gtgtccaaaa aaggattctg acagctttaa aattaggcct ctgggcttta cggaggaagt
     1441 agaggttatt cttcagtaca tctgtgaatg tgaatgccaa agcgaaggca tccctgaaag
     1501 tcccaagtgt catgaaggaa atgggacatt tgagtgtggc gcgtgcaggt gcaatgaagg
     1561 gcgtgttggt agacattgtg aatgcagcac agatgaagtt aacagtgaag acatggatgc
     1621 ttactgcagg aaagaaaaca gttcagaaat ctgcagtaac aatggagagt gcgtctgcgg
     1681 acagtgtgtt tgtaggaaga gggataatac aaatgaaatt tattctggca aattctgcga
     1741 gtgtgataat ttcaactgtg atagatccaa tggcttaatt tgtggaggaa atggtgtttg
     1801 caagtgtcgt gtgtgtgagt gcaaccccaa ctacactggc agtgcatgtg actgttcttt
     1861 ggatactagt acttgtgaag ccagcaacgg acagatctgc aatggccggg gcatctgcga
     1921 gtgtggtgtc tgtaagtgta cagatccgaa gtttcaaggg caaacgtgtg agatgtgtca
     1981 gacctgcctt ggtgtctgtg ctgagcataa agaatgtgtt cagtgcagag ccttcaataa
     2041 aggagaaaag aaagacacat gcacacagga atgttcctat tttaacatta ccaaggtaga
     2101 aagtcgggac aaattacccc agccggtcca acctgatcct gtgtcccatt gtaaggagaa
     2161 ggatgttgac gactgttggt tctattttac gtattcagtg aatgggaaca acgaggtcat
     2221 ggttcatgtt gtggagaatc cagagtgtcc cactggtcca gacatcattc caattgtagc
     2281 tggtgtggtt gctggaattg ttcttattgg ccttgcatta ctgctgatat ggaagctttt
     2341 aatgataatt catgacagaa gggagtttgc taaatttgaa aaggagaaaa tgaatgccaa
     2401 atgggacacg ggtgaaaatc ctatttataa gagtgccgta acaactgtgg tcaatccgaa
     2461 gtatgaggga aaaggtagcg catccggggg tgcgtcaggc ggcagcggat ctggaccggt
     2521 cgccaccatg agtgcgatta agccagacat gaagatcaac ctccgtatgg aaggcaacgt
     2581 aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg agggaaaaca
     2641 gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac
     2701 cactgcattc cattacggca acagggtatt cgccgaatat ccagaccaca tacaagacta
     2761 ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg
     2821 gggcatttgc attgccagaa acgacataac aatggaaggg gacactttct ataataaagt
     2881 tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa
     2941 atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgactg gtgatattcg
     3001 catggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa
     3061 agctaaggag aagggtgtca agttaccagg ctaccacttt gtggaccact gcattgagat
     3121 tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc
     3181 tggattgcct gacaatgcca gacgatccgg tagcggtcac ggtactggtt ctactggttc
     3241 tggttcttct gagagtgcga ttaagccaga catgaagatc aacctccgta tggaaggcaa
     3301 cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa
     3361 acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct
     3421 gaccactgca ttccattacg gcaacagggt attcgccgaa tatccagacc acatacaaga
     3481 ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga
     3541 cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt tctataataa
     3601 agttcgattt cacggtgtaa actttcccgc caatggtcca gttatgcaga agaagacgct
     3661 gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga ctggtgatat
     3721 tcgcatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta
     3781 caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc actgcattga
     3841 gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca
     3901 ttctggattg cctgacaatg ccagacgata agcggccgcg actctagatc ataatcagcc
     3961 ataccacat
//
