LOCUS       sequence-109645-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N4-R5-RNRV RNAP mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Meyer AJ, Ellefson JW, Ellington AD
  TITLE     Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase 
            Variants for in Vivo or in Vitro Synthetic Circuitry.
  JOURNAL   ACS Synth Biol. 2014 Oct 7.
  PUBMED    25279711
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Oct 7."
COMMENT     Sequence Label: pQE-WT-N4-R5-RNRV
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attactggat ctatcaacag gagtccaagc tcagctaatt aagcttttac gcgaacgcga
       61 agtccgactc taagatgtca cggaggttca agttaccttt agccggaagt gctggcattt
      121 tgtccaattg agactcgtgc aactggtcag cgaactggtc gtagaaatca gccagtacat
      181 cacaagactc atatgtgtca accatagttt cgcgcactgc tttgaacagg ttcgcagcgt
      241 cagccggaat ggtaccgaag gagtcgtgaa tcagtgcaaa agattcgatt ccgtacttct
      301 cgtgtgccca cactacagtc ttacgaaggt ggctaccgtc ttggctgtgt acaaagttag
      361 gagcgatacc agacacctgt ttgcgtgcat taatctcgct atctttgttg gtgttaatgg
      421 tcagcgccat gcgacgggta ccgaggatca cgcagtcaat gcgcgtctga ataggcttct
      481 tgtattcctg ccacacaggg aaaccatcag gagttaccca atgcacagcg caacgcttgc
      541 gaagaatctc tccagtcttc ttatctttga cctcagcagc cagcagctta gcagcagact
      601 taagccagtt cattgcttca accgcagcta ccaccgtcac gctcacagat tcccaaatca
      661 gcttagccat gtatccagca gcctgattcg gctgagtgaa catcagaccc ttgccggaat
      721 caatagctgg ctgaatggta tcttccagca cttgttgacg gaagccgaac tctttggacc
      781 cgtaagccag cgtcatgact gaacgcttag tcacactgcg agtaacaccg taagccagcc
      841 attgaccagc cagtgcctta gtgcccagct tgactttctc agagatttca ccagtgttct
      901 catcggtcac ggtaactact tcgttatcgg t
//
