LOCUS       sequence-109656-        9676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of murine CDS encoding for NfiB. Toghther with 
            TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Caiazzo M, Giannelli S, Valente P, Lignani G, Carissimo A, Sessa A, 
            Colasante G, Bartolomeo R, Massimino L, Ferroni S, Settembre C, 
            Benfenati F, Broccoli V
  TITLE     Direct conversion of fibroblasts into functional astrocytes by 
            defined transcription factors.
  JOURNAL   Stem Cell Reports. 2015 Jan 13;4(1):25-36. doi: 
            10.1016/j.stemcr.2014.12.002. Epub 2014 Dec 31.
  PUBMED    25556566
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.12.002"; journalName: "Stem Cell Reports"; 
            date: "2015-01-13- 13"; volume: "4"; issue: "1"; pages: "25-36"
COMMENT     Sequence Label: TetO-FUW-NfiB
FEATURES             Location/Qualifiers
     source          1..9676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2723
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4137..4725
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4190..4210)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4608..4619)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4728..4744)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4729..4745)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5250..5430
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5456..5473)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5462..5686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5732..6160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5819..5838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6029..6050
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6169..6189)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6174..6503
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6354..6489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6416..6435
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6551..6598
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6617..6991
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7121..7242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7158..7177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7212..7231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7291..7307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7291..7307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7304..7326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7315..7331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7339..7345,7346..7363,7364..7369))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7384..7405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7522..7539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7693..8281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7773..7792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8452..9243,9244..9312))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9075..9094
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9313..9417)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9492..9511)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     2821 tcgttaacgg atccatgatg tattctccca tctgtctcac tcaggatgaa ttccacccat
     2881 ttattgaggc acttcttcct cacgtccgtg caattgccta tacttggttc aacctgcagg
     2941 ctcgaaaacg caagtacttt aaaaagcatg agaaacgaat gtcgaaggat gaagaaaggg
     3001 cagtcaaaga cgagctgctc agtgagaagc ccgaaatcaa gcagaagtgg gcatccaggc
     3061 tcctggccaa actgcgcaaa gatatccgcc aggagtaccg ggaggacttt gtgcttaccg
     3121 tgactggcaa gaagcacccg tgctgtgtct tatccaatcc agaccagaag ggtaagatta
     3181 ggaggatcga ctgcctgcga caggcagaca aagtctggcg tctggatcta gtcatggtga
     3241 tcctgttcaa aggcatccct ttggagagta cggatggaga gcgactcatg aagtccccgc
     3301 actgcacaaa cccagcactt tgtgttcagc cacaccacat cacagtatca gttaaggagc
     3361 ttgacttgtt tttggcatac tacgtgcagg agcaagattc tggacaatca ggaagtccaa
     3421 gccacagtga tcctgccaag aatcctccag ggtacctcga ggacagcttt gtaaaatccg
     3481 gagtcttcaa tgtatcagag cttgtgagag tatccagaac acccataacc cagggaactg
     3541 gagtcaactt cccaatcgga gaaattccca gccaaccata ctatcatgac atgaactctg
     3601 gtgtgaacct gcagaggtcg ctgtcttctc caccgagcag caaaagaccc aaaactatat
     3661 ctatagatga aaatatggag ccaagtccta caggagactt ttacccctct ccaaattcac
     3721 cagctgctgg aagtcgaaca tggcatgaac gagatcaaga tatgtcttct ccaactacaa
     3781 tgaagaagcc tgagaagcca ctgtttagct ctacatctcc acaggattct tccccaagat
     3841 tgagcacttt cccccagcac catcatcccg gaatacctgg agtcgcgcac agtgtcatct
     3901 caactcgaac tccacctccg ccctcaccgt tgccatttcc gacgcaagct atccttcctc
     3961 cggcaccttc cagctacttc tctcatccaa caatcagata tcctcctcac ctgaatcctc
     4021 aggatactct gaagaactac gtaccttctt atgacccatc cagtcctcaa acgagccagt
     4081 cctggtacct gggctagtct agataaagcg ctcaattcga tatcaagctt atcgataatc
     4141 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4201 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4261 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4321 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4381 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4441 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4501 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4561 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4621 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4681 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     4741 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     4801 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     4861 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4921 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     4981 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     5041 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     5101 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     5161 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     5221 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     5281 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5341 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5401 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     5461 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5521 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5581 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5641 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5701 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5761 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5821 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5881 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5941 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6001 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6061 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6121 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     6181 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6241 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6301 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6361 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6421 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6481 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6541 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     6601 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     6661 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     6721 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     6781 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     6841 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     6901 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     6961 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     7021 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7081 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7141 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7201 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7261 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7381 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7441 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7501 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7561 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7621 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7681 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7741 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8041 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8101 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8161 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8221 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8281 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8341 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8401 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8461 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8521 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8581 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8641 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8701 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8761 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8821 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8881 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8941 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9001 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9061 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9121 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9181 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9241 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9301 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9361 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9421 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     9481 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     9541 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     9601 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     9661 gtacgggcca gatata
//
