LOCUS       sequence-109675-        8395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasma membrane-targeted FRET biosensor for monitoring calcineurin 
            (CaN) activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3.1(+)-PM-CaNARi
FEATURES             Location/Qualifiers
     source          1..8395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(946..948,949..951,952..1662)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3238..3240,3241..3243,3244..3957)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3989..4006)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3995..4219
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4265..4693
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4352..4371)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4562..4583
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4702..4722)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4707..5036
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4887..5022
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4949..4968
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5103..5897
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5157..5176)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5767..5786
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6071..6192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6108..6127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6162..6181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6241..6257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6241..6257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6254..6276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6265..6281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6289..6295,6296..6313,6314..6319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6334..6355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6472..6489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6643..7228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6723..6742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7399..8190,8191..8259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8022..8041
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8260..8364)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgg gctgcatcaa gagcaagcgc aaggacaaga agcttatggt gagcaagggc
      961 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1021 cacaggttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1081 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1141 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1201 aagtccgcca tgcccgaagg ctacgtccag gagcgtacca tcttcttcaa ggacgacggc
     1261 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1321 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1381 gccatcagtg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggcccac
     1441 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1501 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1561 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1621 accgccgccg ggatcactct cggcatggac gagctgtaca agggatccat gtccgagccc
     1681 aaggcaattg atcccaagtt gtcgacgacc gacagggtgg tgaaagctgt tccatttcct
     1741 ccaagtcacc ggcttacagc aaaagaagtg tttgataatg atggaaaacc tcgtgtggat
     1801 atcttaaagg cgcatcttat gaaggaggga aggctggaag agagtgttgc attgagaata
     1861 ataacagagg gtgcatcaat tcttcgacag gaaaaaaatt tgctggatat tgatgcgcca
     1921 gtcactgttt gtggggacat tcatggacaa ttctttgatt tgatgaagct ctttgaagtc
     1981 gggggatctc ctgccaacac tcgctacctc ttcttagggg actatgttga cagagggtac
     2041 ttcagtattg aatgtgtgct gtatttgtgg gccttgaaaa ttctctaccc caaaacactg
     2101 tttttacttc gtggaaatca tgaatgtaga catctaacag agtatttcac atttaaacaa
     2161 gaatgtaaaa taaagtattc agaacgagta tatgatgcct gtatggatgc ctttgactgc
     2221 cttcccctgg ctgccctgat gaaccaacag ttcctgtgtg tgcatggtgg tttgtctcca
     2281 gagattaaca ctttagatga tatcagaaaa ttagaccgat tcaaagaacc acctgcatat
     2341 ggacctatgt gtgatatcct gtggtcagac cccctggaag attttggaaa tgagaagact
     2401 caggaacatt tcactcacaa cacagtcagg gggtgttcat acttctacag ttacccggct
     2461 gtatgtgaat tcttacagca caataacttg ttatctatac tccgagccca cgaagcccaa
     2521 gatgcagggt accgcatgta caggaaaagc caaacaacag gcttcccttc tctaattaca
     2581 attttttcag caccaaatta cttagatgta tacaataaca aagctgcagt attgaagtat
     2641 gagaacaatg ttatgaatat caggcaattc aactgttctc ctcatccata ctggcttcca
     2701 aatttcatgg atgtttttac ttggtccctt ccatttgttg gggaaaaagt gactgagatg
     2761 ctggtaaatg tcctcaacat ctgctcagat gatgaactag ggtcagaaga agatggattt
     2821 gatggtgcaa cagctgcagc ccggaaagag gtgataagga acaagatccg agcaataggc
     2881 aaaatggcca gagtgttctc agtgctcaga gaagagagtg agagtgtgct gacgctgaaa
     2941 ggcttgaccc caactggcat gctccccagc ggagtacttt ctggagggaa gcaaaccctg
     3001 caaagcgcta ctgttgaggc tattgaggct gatgaagcta tcaaaggatt ttcaccacaa
     3061 cataagatca ctagcttcga ggaagccaag ggcttagacc gaattaatga gaggatgccg
     3121 cctcgcagag atgccatgcc ctctgacgcc aaccttaact ccatcaacaa ggctctcacc
     3181 tcagagacta acggcacgga cagcaatggc agtaatagca gcaatattca gctcgagatg
     3241 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3301 gacgtaaacg gccacaagtt cagcgtgtcc ggtgagggcg agggcgatgc cacctacggc
     3361 aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3421 gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     3481 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     3541 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3601 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3661 ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     3721 atcaaggcca actttaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     3781 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3841 ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3901 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatct
     3961 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     4021 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4081 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4141 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     4201 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     4261 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4321 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4381 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4441 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4501 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4561 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4621 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4681 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4741 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4801 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4861 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4921 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4981 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5041 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     5101 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5161 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     5221 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     5281 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     5341 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     5401 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     5461 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     5521 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5581 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     5641 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5701 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5761 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5821 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5881 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5941 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     6001 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     6061 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6121 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6181 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     6241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7081 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     7201 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7261 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7321 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7381 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7441 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7501 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7561 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7621 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7681 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7741 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7801 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7861 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7921 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7981 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8041 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8101 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8161 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8221 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8281 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8341 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
