LOCUS       sequence-10968-e       12799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie 
            TW, Helmchen F, Denk W, Brecht M, Osten P
  TITLE     Lentivirus-based genetic manipulations of cortical neurons and their
            optical and electrophysiological monitoring in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Dec 28. 101(52):18206-11.
  PUBMED    15608064
REFERENCE   2  (bases 1 to 12799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Dec 28. 101(52):18206-11."
COMMENT     Sequence Label: pFThGW
FEATURES             Location/Qualifiers
     source          1..12799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      6725..6734
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6731..6733,6734..6736,6737..7450)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6731..6733,6734..6736,6737..7447)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6776..6797)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7037..7056)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7384..7405
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7491..8079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7544..7564)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7962..7973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8082..8098)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8083..8099)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8604..8784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8810..8827)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8816..9040
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9086..9514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9173..9192)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9383..9404
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9523..9543)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9528..9857
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9708..9843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9770..9789
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9905..9952
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9971..10345
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10475..10596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10512..10531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10566..10585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10645..10661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10645..10661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10658..10680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10669..10685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10693..10699,10700..10717,10718..10723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10738..10759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10876..10893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11047..11635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11127..11146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11806..12597,12598..12666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12429..12448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12667..12771)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaaatt cagagaccgg gaaccaaact agcctttaaa aaacataagt
     2641 acaggagcca gcaagatggc tcagtgggta aaggtgccta ccagcaagcc tgacagcctg
     2701 agttcagtcc ccacgaacta cgtggtagga gaggaccaac caactctgga aatctgttct
     2761 gcaaacacat gctcacacac acacacacaa atagtataaa caattttaaa tttcatttaa
     2821 aaataatttg taaacaaaat cattagcaca ggttttagaa agagcctctt ggtgacatca
     2881 agttgatgct gtagatgggg tatcattcct gaggacccaa aaccgggtct cagcctttcc
     2941 ccattctgag agttctctct tttctcagcc actagctgaa gagtagagtg gctcagcact
     3001 gggctcttga gttcccaagt cctacaactg gtcagcctga ctactaacca gccatgaaga
     3061 aacaaggagt ggatgggctg agtctgctgg gatgggagtg gagttagtaa gtggccatgg
     3121 atgtaatgac cccagcaatg ctggctagaa ggcatgcctc ctttccttgt ctggagacgg
     3181 aacgggaggg atcatcttgt actcacagaa gggagaacat tctagctggt tgggccaaaa
     3241 tgtgcaagtt cacctggagg tggtggtgca tgcttttaac tccagtactc aggaggcagg
     3301 gccaggtgga tctctgtgag ttcaagacca gcctgcacta tggagagagt tttgggacag
     3361 ccagagttac acagaaaaat cctggtggaa aatctgaaag aaagagagaa agaaagaaag
     3421 aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa ggaaggaagg
     3481 aaggaaggaa ggaaggaagg aaggaaggaa aataggtgcg acttcaagat ccggagttac
     3541 aagcagaatg cactgtttcc ctaacagggc caagtgtttt gagtaactga aggtgggcat
     3601 gatgcctagg aagcgaaaca agccaagcag atgcacccct tgcctttctt ccgaagggct
     3661 gcagtagcat ggaaaagatg gaaaacaacc aatccattcc ctttgctgat ataacaggct
     3721 ccaaagccaa aacctgtcac tggaggctca agagcagatc tccagccaag aggcaaagga
     3781 atgggggaag ctggagggcc tccctctggt tatccaggct tctgaaggtt caagcaaaga
     3841 aagggttaca accttaaaag gagagcgtcc cggggtatgg gtagaagact gctccacccc
     3901 gacccccagg gtccctaacc gtcttttcct tgggcgagtc agcccaatca caggactgag
     3961 agtgcctctt tagtagcagc aagccacttc ggacacccaa atggaacacc tccagtcagc
     4021 cctcgccgac caccccaccc cctccatcct tttccctcag cctccgattg gctgaatcta
     4081 gagtccctcc ctgctccccc ctctctcccc acccctggtg aaaactgcgg gcttcagcgc
     4141 tgggtgcagc aactggaggc gttggcgcac caggaggagg ctgcagctag gggagtccag
     4201 gtgagagcag gccgacggga gggacccgca catgcaagga ccgccgcagg gcgaggatgc
     4261 aagccttccc cagctacagt tttgggaaag gataccaggg cgctcctata tgggggcgcg
     4321 ggaactgggg aaagaaggtg ctcccaggtc gaggtgggag aggaaggcag tgcggggtca
     4381 cgggctttct ccctgctaac ggacgctttc gaagagtggg tgccggagga gaaccatgag
     4441 gaaggacatc aaggacagcc tttggtcccc aagctcaaat cgctttagtg gtgcgaatag
     4501 agggaggagg tgggtggcaa actggaggga gtccccagcg ggtgacctcg tggctggctg
     4561 ggtgcggggc accgcaggta agaaaaccgc aatgttgcgg gaggggactg ggtggcaggc
     4621 gcgggggagg ggaaagctag aaaggatgcg agggagcgga ggggggaggg agcgggagaa
     4681 tctcaactgg tagaggaaga ttaaaatgag gaaatagcat cagggtgggg ttagccaagc
     4741 cgggcctcag ggaaagggcg caaagtttgt ctgggtgtgg gcttaggtgg gctgggtatg
     4801 agattcgggg cgccgaaaac actgctgcgc ctctgccaaa tcacgctacc cctgtatcta
     4861 gttctgccag gcttctccag ccccagcccc aattcttttc tctagtgtcc ccttccctcc
     4921 cctgaatctc aagcccacac tccctcctcc ataacccact gttatcaaat ctaagtcatt
     4981 tgccacccaa caaccatcag gaggcggaag cagacgggag gagtttgaga tcaacttggg
     5041 ctacatcacg agttccaggc tcaccaaggc ttcttaagga gaccttgtct ctaaaattaa
     5101 ttaattaatt aattaatagt cccctttctc tgccacagaa ccttgggatc tggctcctgg
     5161 tcgcagctcc ccccacccca ggctgacatt cactgccata gcccatccgg aaatcctagt
     5221 ctatttcccc atggatcttg aactgcagag agaatggcag agtggcccgc cctgtgcaaa
     5281 ggatgttcct agcctaggtg gagctcgcga actcgcagac tgtgcctctc ttgggcaagg
     5341 acaggctaga cagcctgccg gtgtgttgag ctagggcact gtggggaagg cagagaacct
     5401 gtgcagggca gcaatgaaca caggaccaga aaactgcagc cctaggaaca ctcaagagct
     5461 ggccatttgc aagcatctct ggcctccgtg cttctcactc atgtcccatg tcttatacag
     5521 gcctctgtgg cacctcgctt gcctgatctc atccctagcc gttaagcttt ctgcatgact
     5581 tatcacttgg ggcataatgc tggataccta ccattttctt agaccccatc aaaatcctat
     5641 ttgagtgtac ggttcggaga acctcattta tccggtaaat gtcttttact ctgctctcag
     5701 ggagctgagg caggacatcc tgagatacat tgggagagga gatacagttt caataaaata
     5761 ataggttggg tggaggtaca tgcctataat gccaccactc aggaaatggt ggcagcttcg
     5821 tgagtttgag gccaacccaa gaaacatagt gaaaccctgt cagtaaataa gtaagcaagt
     5881 atttgagtat ctactatatg ctagggctga cctggacatt aggggtcatc ttctgaacaa
     5941 actagtgctt gagggaggta tttggggttt ttgtttgttt aatggatctg aatgagttcc
     6001 agagactggc tacacagcga tatgactgag cttaacaccc ctaaagcata cagtcagacc
     6061 aattagacaa taaaaggtat gtatagctta ccaaataaaa aaattgtatt ttcaagagag
     6121 tgtctgtctg tgtagccctg gctgttcttg aactcactct gtagaccagg ctggcctgga
     6181 aatccatctg cctgcctctg cctctctgcc tctctgcctc tctgcctctc tctctgcctc
     6241 tctctgcctc tctctgcccc tctctgcccc tctctgcccc tctctgccgc cctctgcctt
     6301 ttgccctctg ccctctgttc tctggcctct gccctctgcc ctctggcctc tggcctctgc
     6361 ctctgcctct tgagtgctgg aatcaaaggt gtgagctctg taggtcttaa gttccagaag
     6421 aaagtaatga agtcacccag cagggaggtg ctcagggaca gcacagacac acacccagga
     6481 cataggctcc cacttccttg gctttctctg agtggcaaag gaccttaggc agtgtcactc
     6541 cctaagagaa ggggataaag agaggggctg aggtattcat catgtgctcc gtggatctca
     6601 agccctcaag gtaaatgggg acccacctgt cctaccagct ggctgacctg tagctttccc
     6661 caccacagaa tccaagtcgg aactcttggc acctagagga tctcgagatc cccgggtacc
     6721 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     6781 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     6841 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     6901 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     6961 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     7021 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     7081 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     7141 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     7201 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     7261 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     7321 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     7381 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     7441 gtacaagtaa agcggccgcg actctagaat tcgatatcaa gcttatcgat aatcaacctc
     7501 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7561 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7621 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7681 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7741 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7801 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7861 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7921 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7981 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     8041 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     8101 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     8161 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     8221 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8281 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     8341 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     8401 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     8461 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     8521 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     8581 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8641 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8701 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8761 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     8821 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8881 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8941 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     9001 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     9061 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9481 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     9541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9601 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9661 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     9721 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9781 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9841 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     9901 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     9961 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
    10021 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
    10081 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    10141 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    10201 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    10261 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    10321 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    10381 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    10441 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    10501 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10561 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    10621 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    10681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    10741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    10801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    10861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    10921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    11521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    11641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    11701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    11761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    11821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    11881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    11941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    12421 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    12481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    12541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    12601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    12661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    12721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    12781 ccgaaaagtg ccacctgac
//
