LOCUS       sequence-109699-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains Plac driving expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZA2[PLac:GFP]
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     183..201
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    224..240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    247..263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             305..316
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
       61 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
      121 gcgccccagc tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata
      181 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaattgtgag cggataacaa
      241 ttgacattgt gagcggataa caagatactg agcacatcag caggacgcac tgaccgaatt
      301 cattaaagag gagaaaggta ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc
      361 aattttggtt gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg
      421 tgaaggtgat gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt
      481 gccagttcca tggccaacct tagtcactac tttaacttat ggtgttcaat gtttttctag
      541 atacccagat catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt
      601 tcaagaaaga actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa
      661 gtttgaaggt gataccttag ttaatagaat cg
//
