LOCUS       sequence-109700-        2025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with minCMV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_minCMV
FEATURES             Location/Qualifiers
     source          1..2025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..85
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     rep_origin      complement(289..877)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(369..388)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1048..1839,1840..1908))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1671..1690
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1909..2013)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattacgcgt aactcgagta tgtcgaggtg gcgtgtacgg tgggaggcct atataagcag
       61 agctcgttta gtgaaccgtc agatcgcctg gagaattcag atctcagctg gtcgaccatg
      121 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      181 ggcggtaata cggttatata cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      241 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      301 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      361 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      421 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      481 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      541 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      601 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      661 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      721 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      781 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      841 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      901 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      961 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1021 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1081 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1141 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1201 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1261 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1321 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1381 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1441 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1501 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1561 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1621 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1681 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1741 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1801 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     1861 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     1921 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     1981 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccg
//
