LOCUS       sequence-109704-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PTAN driving expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZA2[PTAN:GFP]
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     183..201
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             304..315
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
       61 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
      121 gcgccccagc tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata
      181 aaaataggcg tatcacgagg ccctttcgtc ttcacctgag aattttaagc ggcttaaaat
      241 tgacatttta agcgcttaaa atgatactga gcacatcagc aggacgcact gaccgaattc
      301 attaaagagg agaaaggtac catgtctaaa ggtgaagaat tattcactgg tgttgtccca
      361 attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt
      421 gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac tggtaaattg
      481 ccagttccat ggccaacctt agtcactact ttaacttatg gtgttcaatg tttttctaga
      541 tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga aggttatgtt
      601 caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc tgaagtcaag
      661 tttgaaggtg atacctta
//
