LOCUS       sequence-109712-        8697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lysosome-targeted FRET biosensor for monitoring Akt activity; 
            targeted to cytosolic face of lysosomal membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-Lyso-AktAR2
FEATURES             Location/Qualifiers
     source          1..8697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      905..914
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(907..990,991..2157)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     CDS             join(2182..2184,2185..2187,2188..2898)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3451..4185
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     promoter        complement(4249..4267)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4250..4267)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4287..4304)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4293..4517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4563..4991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4650..4669)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4860..4881
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5000..5020)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5005..5335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5186..5321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5248..5267
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5402..6196
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5456..5475)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6066..6085
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6370..6491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6407..6426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6461..6480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6540..6556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6540..6556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6553..6575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6564..6580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6588..6594,6595..6612,6613..6618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6633..6654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6771..6788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6942..7530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7022..7041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7701..8492,8493..8561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8324..8343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8562..8666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg cggcccccgg cagcgcccgg cgacccctgc tgctgctact gctgttgctg
      961 ctgctcggcc tcatgcattg tgcgtcagca gcaatgttta tggtgaaaaa tggcaacggg
     1021 accgcgtgca taatggccaa cttctctgct gccttctcag tgaactacga caccaagagt
     1081 ggccctaaga acatgacctt tgacctgcca tcagatgcca cagtggtgct caaccgcagc
     1141 tcctgtggaa aagagaacac ttctgacccc agtctcgtga ttgcttttgg aagaggacat
     1201 acactcactc tcaatttcac gagaaatgca acacgttaca gcgtccagct catgagtttt
     1261 gtttataact tgtcagacac acaccttttc cccaatgcga gctccaaaga aatcaagact
     1321 gtggaatcta taactgacat cagggcagat atagataaaa aatacagatg tgttagtggc
     1381 acccaggtcc acatgaacaa cgtgaccgta acgctccatg atgccaccat ccaggcgtac
     1441 ctttccaaca gcagcttcag caggggagag acacgctgtg aacaagacag gccttcccca
     1501 accacagcgc cccctgcgcc acccagcccc tcgccctcac ccgtgcccaa gagcccctct
     1561 gtggacaagt acaacgtgag cggcaccaac gggacctgcc tgctggccag catggggctg
     1621 cagctgaacc tcacctatga gaggaaggac aacacgacgg tgacaaggct tctcaacatc
     1681 aaccccaaca agacctcggc cagcgggagc tgcggcgccc acctggtgac tctggagctg
     1741 cacagcgagg gcaccaccgt cctgctcttc cagttcggga tgaatgcaag ttctagccgg
     1801 tttttcctac aaggaatcca gttgaataca attcttcctg acgccagaga ccctgccttt
     1861 aaagctgcca acggctccct gcgagcgctg caggccacag tcggcaattc ctacaagtgc
     1921 aacgcggagg agcacgtccg tgtcacgaag gcgttttcag tcaatatatt caaagtgtgg
     1981 gtccaggctt tcaaggtgga aggtggccag tttggctctg tggaggagtg tctgctggac
     2041 gagaacagca tgctgatccc catcgctgtg ggtggtgccc tggcggggct ggtcctcatc
     2101 gtcctcatcg cctacctcgt cggcaggaag aggagtcacg caggctacca gactatcggt
     2161 ggtggcggtg gtgcggatcc catggtgagc aagggcgagg agctgttcac cggggtggtg
     2221 cccatcctgg tcgagctgga cggcgacgta aacggccaca ggttcagcgt gtccggcgag
     2281 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2341 ctgcccgtgc cctggcccac cctcgtgacc accctgagct ggggcgtgca gtgcttcgcc
     2401 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2461 gtccaggagc gtaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2521 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2581 gacggcaaca tcctggggca caagctggag tacaacgcca tccacggcaa cgtctatatc
     2641 accgccgaca agcagaagaa cggcatcaag gccaacttcg gcctcaactg caacatcgag
     2701 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2761 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2821 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2881 atggacgagc tgtacaagcg catgcataag ttttctcaag aacagatcgg cgaaaacatt
     2941 gtgtgcaggg tcatttgtac cacgggtcaa attcccatcc gagatttgtc agctgatatt
     3001 tcacaagtgc ttaaggaaaa acgatccata aagaaagttt ggacatttgg tagaaaccca
     3061 gcctgtgact atcatttagg aaacatttca agactgtcaa ataagcattt ccaaatacta
     3121 ctaggagaag acggtaacct tttattgaat gacatttcca ctaatgggac ctggttaaat
     3181 gggcaaaaag tcgagaagaa cagcaatcag ttactgtctc aaggtgatga aataaccgtt
     3241 ggtgtaggcg tggaatcaga tattttatct ctggtcattt tcataaacga caaatttaag
     3301 cagtgcctcg agcagaacaa agttgatcgc tctgcaggta agccaggcag cggcgagggc
     3361 agcaccaagg gcctggtcga ccctcgtccg cgctcgtgca cctggccgga ccccaggccg
     3421 gagtttggag gtaccggcgg cagcgagctc atgggcggcg tgcagctcgc cgaccactac
     3481 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3541 taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3601 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aggtaccggt
     3661 ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3721 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3781 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     3841 cccaccctcg tgaccaccct gggctacggc cttcagtgct tcgcccgcta ccccgaccac
     3901 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3961 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4021 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4081 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     4141 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgagtaaga attctgcaga
     4201 tatccatcac actggcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca
     4261 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4321 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4381 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4441 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4501 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4561 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4621 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4681 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
     4741 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4801 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4861 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4921 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4981 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5041 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5101 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5161 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5221 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5281 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5341 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     5401 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5461 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5521 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5581 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5641 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5701 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5761 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5821 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5881 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5941 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6001 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6061 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6121 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6181 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6241 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6301 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6361 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6481 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     6541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6601 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     7141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7381 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
