LOCUS       sequence-109724-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For yeast two hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Tabata K, Matsunaga K, Sakane A, Sasaki T, Noda T, Yoshimori T
  TITLE     Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic 
            pathway via a novel Rab7-binding domain.
  JOURNAL   Mol Biol Cell. 2010 Dec;21(23):4162-72. doi: 
            10.1091/mbc.E10-06-0495. Epub 2010 Oct 13.
  PUBMED    20943950
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E10-06-0495"; journalName: "Mol Biol Cell"; date: 
            "2010-12"; volume: "21"; issue: "23"; pages: "4162-72"
COMMENT     Sequence Label: pGAD-hRubicon
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             164..184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             200..541
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt
       61 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcc
      121 aagctttgca aagatggata aagcggaatt aattcccgag cctccaaaaa agaagagaaa
      181 ggtcgaattg ggtaccgccg ccaattttaa tcaaagtggg aatattgctg atagctcatt
      241 gtccttcact ttcactaaca gtagcaacgg tccgaacctc ataacaactc aaacaaattc
      301 tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat gataacttca tgaataatga
      361 aatcacggct agtaaaattg atgatggtaa taattcaaaa ccactgtcac ctggttggac
      421 ggaccaaact gcgtataacg cgtttggaat cactacaggg atgtttaata ccactacaat
      481 ggatgatgta tataactatc tattcgatga tgaagatacc ccaccaaacc caaaaaaaga
      541 gatcgaattc atgcggccgg agggcgcggg aatggagctc ggaggcggcg aggagcgcct
      601 gcctgaggag agcaggaggg agcactggca gttgctgggt aatttgaaga cgacggtgga
      661 gggtttggta tcaaccaaca gccccaacgt ctggtctaag tatggtggct tggagcggct
      721 ttgcagggac atgcagagca tcctctatca cgggcttatc cgtgaccagg cgtgccgccg
      781 ccagacggat tactggcagt tcgtgaaaga catccggtgg ctcagtcccc actcagccct
      841 tcacgtggag aagttcatca gcgtgcacga gaacgaccag agcagtgctg atggtgccag
      901 tgaacgtgct gttgccgagc tgtggctgca gcaca
//
