LOCUS       sequence-109741-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PLac driving expression of mCherry and PTAN driving 
            expression of GFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZA2[PLac:mCherry,PTAN:GFP]
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             47..58
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    657..673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    680..696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgggacaac accagtgaat aattcttcac ctttagacat ggtacctttc tcctctttaa
       61 tgaattcggt cagtgcgtcc tgctgatgtg ctcagtatca ttttaagcgc ttaaaatgtc
      121 aattttaagc cgcttaaaat tctcgagccc ttaacgtgag ttttcgttcc actgagcgtc
      181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
      241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
      361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      421 cgctctgcta atcctgttac cagtcaggca tttgagaagc acacggtcac actgcttccg
      481 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
      541 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
      601 ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccgcgg ccgcaattgt
      661 gagcggataa caattgacat tgtgagcgga taacaagata ctgagcacat cagcaggacg
      721 cactgaccgg cgcgccagct gtcaccggat gtgc
//
