LOCUS       sequence-109745-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     Davidson Empty backbones
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSNAPf-N1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..104
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..657
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac catggacaaa
      121 gactgcgaaa tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg
      181 tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc
      241 gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc
      301 gcctggctca acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc
      361 ctgcaccacc cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg
      421 aaagtggtga agttcggaga ggtcatcagc tacagccacc tggccgccct ggccggcaat
      481 cccgccgcca ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc
      541 ccctgccacc gggtggtgca gggcgacctg gacgtggggg gctacgaggg cgggctcgcc
      601 gtgaaagagt ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggttaa
      661 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
      721 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
      781 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatt
//
