LOCUS       sequence-109755-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control gRNA (GTCAAGGCACTCTTGCCTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–CgRNA
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      600..609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             606..4709
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             606..4709
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             606..4709
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4722..4742
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4746..4799
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4812..5507
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             5526..5645
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    5779..5900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5816..5835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5870..5889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5907..6362)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6044..6065)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6256..6275
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6389..6493
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6495..6852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6495..6686
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6518..6538)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6703..6838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6887..7681
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6887..7681
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6941..6960)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7551..7570
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7869..7888)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7913..7960
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        8053..8301
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8053..8073
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8224..8243
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8322..8397
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(8409..8433)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      8765..9353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9254..9273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccttg
      601 ccaccatgga caagaagtac tccattgggc tcgatatcgg cacaaacagc gtcggctggg
      661 ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg
      721 atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc ggggagacgg
      781 ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga
      841 tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc
      901 ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct
      961 ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga
     1021 ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg
     1081 cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca
     1141 gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga
     1201 acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat
     1261 cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg
     1321 gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg
     1381 ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc
     1441 tggcccagat cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg
     1501 ccattctgct gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg
     1561 ctagtatgat caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg
     1621 tcagacagca actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct
     1681 acgccggata cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca
     1741 tcttggaaaa aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt
     1801 tgcgcaaaca gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac
     1861 tgcacgctat cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa
     1921 agattgagaa aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa
     1981 attccagatt cgcgtggatg actcgcaaat cagaagagac catcactccc tggaacttcg
     2041 aggaagtcgt ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg
     2101 ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca
     2161 cagtttataa cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat
     2221 tcctgtctgg agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag
     2281 ttaccgtgaa acagctcaaa gaagactatt tcaaaaagat tgaatgtttc gactctgttg
     2341 aaatcagcgg agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga
     2401 aaatcattaa agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca
     2461 ttgtcctcac ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt
     2521 acgctcatct cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat
     2581 gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa
     2641 tcctggattt tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg
     2701 atgactctct cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca
     2761 gtcttcacga gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc
     2821 agaccgttaa ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata
     2881 tcgttatcga gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg
     2941 aaaggatgaa gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac
     3001 acccagttga aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg
     3061 gcagggacat gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg
     3121 atcatatcgt gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa
     3181 gatccgataa aaatagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa
     3241 tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata
     3301 atctgactaa ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa
     3361 ggcagcttgt tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca
     3421 tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga
     3481 agtctaagct ggtctcagat ttcagaaagg actttcagtt ttataaggtg agagagatca
     3541 acaattacca ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca
     3601 aaaaatatcc caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta
     3661 ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt
     3721 acagcaatat tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga
     3781 agcgaccact tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg
     3841 atttcgcgac agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg
     3901 aagtacagac cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc
     3961 tgatcgcacg caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag
     4021 tcgcttacag tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa
     4081 gcgtcaagga actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca
     4141 tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc
     4201 ccaagtactc tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg
     4261 agctgcagaa aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg
     4321 ccagccacta tgaaaagctc aaagggtctc ccgaagataa tgagcagaag cagctgttcg
     4381 tggaacaaca caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa
     4441 gagtgatcct cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg
     4501 ataagcccat cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg
     4561 gcgcgcctgc agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta
     4621 caaaggaggt cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa
     4681 gaatcgacct ctctcagctc ggtggagaca gcagggctga ccccaagaag aagaggaagg
     4741 tggctgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctt
     4801 ccggagggcc catggtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     4861 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg
     4921 agggcaccca gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc ttcgccttcg
     4981 acatcctggc taccagcttc atgtacggca gcaaaacctt catcaaccac acccagggca
     5041 tccccgactt ctttaagcag tccttccccg agggcttcac atgggagaga gtcaccacat
     5101 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     5161 tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga
     5221 aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc cgctgacggc ggcctggaag
     5281 gcagagccga catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttgaaga
     5341 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg
     5401 acagaagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg
     5461 tggctgtggc cagatactgc gacctcccta gcaaactggg gcacagaggt ggaggaggtt
     5521 ccggatctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc ctgcccatga
     5581 gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc gccaggatca
     5641 acgtctaagg ccggccagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     5701 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     5761 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5881 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     5941 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6001 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6061 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6121 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     6181 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     6241 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     6301 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     6361 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6481 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     6541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6601 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6661 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6721 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6781 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6841 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     6901 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6961 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7021 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     7081 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7141 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7201 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7261 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7321 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7381 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     7441 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7501 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7561 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7621 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7681 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7741 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7801 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     7861 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     7921 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     7981 cagggctggc actctgtcga taccccaccg agacctcagt cgactggatc cggtaccaag
     8041 gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     8101 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
     8161 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     8221 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     8281 ttgtggaaag gacgaaacac cggtcaaggc actcttgcct agttttagag ctagaaatag
     8341 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     8401 ttttctagac ccagctttct tgtacaaagt tggcattaaa gggcgaattc cagcacactg
     8461 gcggccgtta ctagtggatc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     8521 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     8581 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     8641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8881 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9421 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
