LOCUS       sequence-109776-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CYP51A1 (3I3K)
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(136..141,142..159,160..166)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    174..190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(245..250,251..266,267..273)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    281..297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(340..345,346..361,362..368)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    376..392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgctatcatg ccataccgcg gaaaggtttt gcaccattcg atggtgtcct ggcacgacag
       61 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca
      121 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gaattgtgag
      181 cggataacaa tttcacacag gaaacaggat cgatccatcg atgagcttac tccccatccc
      241 cctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
      301 cacacaggaa acaggatcag cttactcccc atccccctgt tgacaattaa tcatcggctc
      361 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagg atccatcgat
      421 gcttaggagg tcatatggct aagaaaacgc tgcccgcagg ggtgaaaagt cctccataca
      481 ttttctcccc aattccattc cttgggcatg ccatagcatt tgggaaaagt ccaattgaat
      541 ttctagaaaa tgcatatgag aagtatggac ctgtatttag ttttaccatg gtaggcaaga
      601 catttactta ccttctgggg agtgatgctg ctgcactgct ttttaatagt aaaaatgaag
      661 acctgaatgc agaagatgtc tacagtcgcc tgacaacacc tgtgttt
//
