LOCUS       sequence-109785-         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and a non-silencing (scrambled) shRNA module. Compatible 
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 n.s shRNA L5-L2
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(175..423)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(233..252)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(403..423)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(466..486)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttctt ataatgccaa
       61 ctttgtacaa gaaagctggg tacgaattca ctgccatttg tctcgaggtc gagaattcaa
      121 aaacctaagg ttaagtcgcc ctcgctcgag cgagggcgac ttaaccttag gaccggtgtt
      181 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
      241 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      301 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattccaat
      361 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      421 ctcggtgaag ggggcggccg ctcgaggcta gtctcggatc gatagcaact tttgtataca
      481 aagttggcat tataaaaaag cattgctcat caatttgttg caacgaacag gtcactatca
      541 gtc
//
