LOCUS       sequence-109788-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA against human pro-caspase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Doitsh G, Galloway NL, Geng X, Yang Z, Monroe KM, Zepeda O, Hunt PW,
            Hatano H, Sowinski S, Munoz-Arias I, Greene WC
  TITLE     Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 
            infection.
  JOURNAL   Nature. 2014 Jan 23;505(7484):509-14. doi: 10.1038/nature12940.
  PUBMED    24356306
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-01-23- 23"; volume: "505"; issue: "7484"; 
            pages: "509-14"
COMMENT     Sequence Label: shRNA Caspase 1 (pSicoR mCherry)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    77..110
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(408..427)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     677..695
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttttgtatcc agaggttgat tatcgataag cttgatatcg aattgtacct agtggaaccg
       61 gaacccttaa acatgtataa cttcgtataa tgtatgctat acgaagttat taggtccctc
      121 gacgaattct tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg
      181 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt
      241 gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
      301 agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc
      361 gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
      421 tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
      481 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
      541 gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg
      601 gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa
      661 gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
      721 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
      781 cttgaa
//
