LOCUS       sequence-109791-         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 
            (aa 2-2414) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-FLp300
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcaaagttg ggcgcgccgc cgagaatgtg gtggaaccgg ggccgccttc agccaagcgg
       61 cctaaactct catctccggc cctctcggcg tccgccagcg atggcacaga ttttggctct
      121 ctatttgact tggagcacga cttaccagat gaattaatca actctacaga attgggacta
      181 accaatggtg gtgatattaa tcagcttcag acaagtcttg gcatggtaca agatgcagct
      241 tctaaacata aacagctgtc agaattgctg cgatctggta gttcccctaa cctcaatatg
      301 ggagttggtg gcccaggtca agtcatggcc agccaggccc aacagagcag tcctggatta
      361 ggtttgataa atagcatggt caaaagccca atgacacagg caggcttgac ttctcccaac
      421 atggggatgg gcactagtgg accaaatcag ggtcctacgc agtcaacagg tatgatgaac
      481 agtccagtaa atcagcctgc catgggaatg aacacaggga tgaatgcggg catgaatcct
      541 ggaatgttgg ctgcaggcaa tggacaaggg ataatgccta atcaagtcat gaacgg
//
