LOCUS       sequence-109793-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with CAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_CAG
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(171..275)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(338..357)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(419..441)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
       61 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaat tggtcgagaa
      301 gcttgtcgac gaattctttg ccaaaatgat gagacagcac aataaccagc acgttgccca
      361 ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggctcta gagccgccgg
      421 tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc
      481 gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc
      541 cctccccgcc catttccttc ctgccggcgc cgcaccgctt cg
//
