LOCUS       sequence-109798-       10195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Rose T, Schoenenberger P, Jezek K, Oertner TG
  TITLE     Developmental refinement of vesicle cycling at schaffer collateral 
            synapses.
  JOURNAL   Neuron. 2013 Mar 20;77(6):1109-21. doi: 
            10.1016/j.neuron.2013.01.021.
  PUBMED    23522046
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.01"; journalName: "Neuron"; date: 
            "2013-03-20- 20"; volume: "77"; issue: "6"; pages: "1109-21"
COMMENT     Sequence Label: pMH4-Syn1-Ratio1XsypHy
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        399..846
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      900..909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1455..1464
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1461..2177
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1494..1522)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2117..2137
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2601..2627
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2631..2640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2635..2644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2637..3314
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             3357..4031
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     polyA_signal    4045..4179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4082..4101)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4136..4155
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             7726..7917
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7911..7933)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8019..8159
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8074..8093
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8174..8191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8345..8933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8425..8444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9104..9895,9896..9964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9727..9746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9965..10069)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10137..10155
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcccttccag ctctctgccc cttttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tctctagcat cgaagatccc
      361 tcgactctag actcgagctg cagagggccc tgcgtatgag tgcaagtggg ttttaggacc
      421 aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact ggacaagcac
      481 ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg aaacaggatg
      541 cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc gcccccgcct
      601 ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact cgccggtccc
      661 ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga
      721 ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg ctgcggcgcc
      781 ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga
      841 gcgcagtcga attcaagctg ctaggtttaa acgggccctc tagactcgag ctcaagcttg
      901 ccaccatgga cgtggtgaat cagctggtgg ctgggggtca gttccgggtg gtcaaggagc
      961 cccttggctt cgtgaaggtg ctgcagtggg tctttgccat cttcgccttt gctacgtgcg
     1021 gcagctacac cggagagctt cggctgagcg tggagtgtgc caacaagacg gagagtgccc
     1081 tcaacatcga agtcgaattt gagtacccat tcaggctgca ccaagtgtac tttgatgcac
     1141 cctcctgcgt taaagggggc actaccaaga tcttcctagt tggtgactac tcctcctcgg
     1201 ctgaattctt tgtcaccgtg gctgtgtttg ccttcctcta ctccatgggg gccctggcca
     1261 cctacatctt cctgcagaac aagtaccgag agaacaacaa agggccaatg atggacttcc
     1321 tggccacagc agtgttcgct ttcatgtggc tagttagctc atccgcctgg gccaaaggcc
     1381 tgtccgatgt gaagatggcc actgacccag agaacattat caaggagatg cctatgtgcc
     1441 gccagacaga tctagccacc atgggaagta aaggagaaga acttttcact ggagttgtcc
     1501 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     1561 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     1621 tacctgttcc ttggccaaca cttgtcacta ctttaactta tggtgttcaa tgcttttcaa
     1681 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
     1741 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     1801 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     1861 atggaaacat tcttggacac aaattggaat acaactataa cgatcaccag gtgtacatca
     1921 tggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattagacac aacattgaag
     1981 atggaggcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatgggcccg
     2041 tccttttacc agacaaccat tacctgttta caacttctac tctttcgaaa gatcccaacg
     2101 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     2161 tggatgaact atacaaaacc ggtggagctc gaatcacatg caaggaactg agggaccctg
     2221 tgacttcagg actcaacacc tcggtggtgt ttggcttcct gaacctggtg ctctgggttg
     2281 gcaacctatg gttcgtgttc aaggagacag gctgggccgc cccattcatg cgcgcacctc
     2341 caggcgcccc agaaaagcaa ccagctcctg gcgatgccta cggcgatgcg ggctatgggc
     2401 agggccccgg aggctatggg ccccaggact cctacgggcc tcagggtggt tatcaacccg
     2461 attacgggca gccagccagc ggcggtggcg gtggctacgg gcctcagggc gactatgggc
     2521 agcaaggcta cggccaacag ggtgcgccca cctccttctc caatcagatg tcggatccga
     2581 agcttcggta ccaagctgcc tacccttacg atgtgcccga ttacgccgcg gccgccatgg
     2641 tggcctcctc cgaggacgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
     2701 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2761 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     2821 tgtcccccca gttccagtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg
     2881 actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2941 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca
     3001 aggtgaagtt ccgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
     3061 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     3121 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct
     3181 acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggacacc aagctggaca
     3241 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc
     3301 accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct
     3361 cctccgagga cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
     3421 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3481 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     3541 cccagttcca gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgactaca
     3601 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3661 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     3721 agttccgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     3781 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     3841 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
     3901 ccaagaagcc cgtgcagctg cccggctact actacgtgga caccaagctg gacatcacct
     3961 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     4021 tgttcctgta ggtcgacttc gagcaacttg tttattgcag cttataatgg ttacaaataa
     4081 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4141 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg tctagcatcg aagatctggg
     4201 cgtggttaag ggtgggaaag aatatataag gtgggggtct tatgtagttt tgtatctgtt
     4261 ttgcagcagc cgccgccgcc atgagcacca actcgtttga tggaagcatt gtgagctcat
     4321 atttgacaac gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg ggctccagca
     4381 ttgatggtcg ccccgtcctg cccgcaaact ctactacctt gacctacgag accgtgtctg
     4441 gaacgccgtt ggagactgca gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg
     4501 ggattgtgac tgactttgct ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat
     4561 ccgcccgcga tgacaagttg acggctcttt tggcacaatt ggattctttg acccgggaac
     4621 ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt
     4681 cctcccctcc caatgcggtt taaaacataa ataaaaaacc agactctgtt tggatttgga
     4741 tcaagcaagt gtcttgctgt ctttatttag gggttttgcg cgcgcggtag gcccgggacc
     4801 agcggtctcg gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa aggtgactct
     4861 ggatgttcag atacatgggc ataagcccgt ctctggggtg gaggtagcac cactgcagag
     4921 cttcatgctg cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt
     4981 gcctaaaaat gtctttcagt agcaagctga ttgccagggg caggcccttg gtgtaagtgt
     5041 ttacaaagcg gttaagctgg gatgggtgca tacgtgggga tatgagatgc atcttggact
     5101 gtatttttag gttggctatg ttcccagcca tatccctccg gggattcatg ttgtgcagaa
     5161 ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg
     5221 cgtggaagaa cttggagacg cccttgtgac ctccaagatt ttccatgcat tcgtccataa
     5281 tgatggcaat gggcccacgg gcggcggcct gggcgaagat atttctggga tcactaacgt
     5341 catagttgtg ttccaggatg agatcgtcat aggccatttt tacaaagcgc gggcggaggg
     5401 tgccagactg cggtataatg gttccatccg gcccaggggc gtagttaccc tcacagattt
     5461 gcatttccca cgctttgagt tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga
     5521 aaacggtttc cggggtaggg gagatcagct gggaagaaag caggttcctg agcagctgcg
     5581 acttaccgca gccggtgggc ccgtaaatca cacctattac cgggtgcaac tggtagttaa
     5641 gagagctgca gctgccgtca tccctgagca ggggggccac ttcgttaagc atgtccctga
     5701 ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc gccgcccagc gatagcagtt
     5761 cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga
     5821 gcgtttgacc aagcagttcc aggcggtccc acagctcggt cacctgctct acggcatctc
     5881 gatccagcat atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg
     5941 gtgctcgtcc agacgggcca gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt
     6001 agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag
     6061 gctggtcctg ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca
     6121 tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc
     6181 cttggaggag gcgccgcacg aggggcagtg cagacttttg agggcgtaga gcttgggcgc
     6241 gagaaatacc gattccgggg agtaggcatc cgcgccgcag gccccgcaga cggtctcgca
     6301 ttccacgagc caggtgagct ctggccgttc ggggtcaaaa accaggtttc ccccatgctt
     6361 tttgatgcgt ttcttacctc tggtttccat gagccggtgt ccacgctcgg tgacgaaaag
     6421 gctgtccgtg tccccgtata cagacttgag aggcctgtcc tcgaccgatg cccttgagag
     6481 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     6541 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     6601 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     6661 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     6721 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     6781 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     6841 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     6901 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca
     6961 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     7021 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     7081 cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     7141 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
     7201 gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg
     7261 ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
     7321 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
     7381 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
     7441 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
     7501 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
     7561 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
     7621 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
     7681 cattaccccc atgaacagaa attccccctt acacggaggc atcaagtgac caaacaggaa
     7741 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     7801 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     7861 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7921 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7981 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     8041 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     8101 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8521 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     8581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8761 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     9421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9721 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10081 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
    10141 ggcgtatcac gaggcccttt cgtcttcaag aattgatccg ggcccccatt tcccc
//
