LOCUS       sequence-109800-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCALSL-mir30
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     347..366
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    365..420
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     466..485
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     ncRNA           complement(708..801)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    839..872
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(905..924)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgggctgca ggtcgaggga tctccataag agaagaggga
       61 cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg
      121 aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac
      181 ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact
      241 gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca
      301 aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc
      361 cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt
      421 agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa
      481 aaagatctca gtggtatttg tgagccaggg cattggccac accagccacc accttctgat
      541 aggcagcctg cacctgagga gtgcggccgc atcgatgata tcaaaagtga taatttatac
      601 cattttaatt cagctttgta aaaatgtatc aaagagatag caaggtattc agttttagta
      661 aacaagataa ttgctcctaa agtagcccct tgaattctgg ttgctcgagc cttctgttgg
      721 gttaacctga agaagtaatc ccagcaagtg tttccaagat gtgcaggcaa cgattctgta
      781 aagtactgaa gcctcattca aacaattacc ctgttatccc tagtcgatcg agactagtat
      841 aacttcgtat aatgtatgct atacgaagtt attcggattt gatccagaca tgataagata
      901 cattgatgag tttggacaaa ccacaactag aatgc
//
