LOCUS       sequence-109801-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCALSL-mir30
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    193..274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    420..501
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    544..678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(687..720)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     ncRNA           758..851
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 tcattttggc aaagaattga ttaattcgag cgaacgcgta ctagtataac ttcgtatagc
       61 atacattata cgagttattt tttgagctcc ataatcagcc ataccacatt tgtagaggtt
      121 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      181 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      241 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      301 atcaatgtat ccgcggcata atcagccata ccacatttgt agaggtttta cttgctttaa
      361 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatatc
      541 gataacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      601 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
      661 tcttatcatg tctggatcaa atccgaataa cttcgtatag catacattat acgaagttat
      721 actagtctcg atcgactagg gataacaggg taattgtttg aatgaggctt cagtacttta
      781 cagaatcgtt gcctgcacat cttggaaaca cttgctggga ttacttcttc aggttaaccc
      841 aacagaaggc tcgagcaacc agaattcaag gggctacttt aggagcaatt atcttgttta
      901 ctaaaactga ataccttgct atctctttga taca
//
