LOCUS       sequence-109802-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for use in AdEasy System. Used for expression of 
            transgenes under a chosen (non-CMV) promoter when no GFP tracer is 
            desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pShuttle
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_signal     147..297
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   385..487
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      525..905
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(612..631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     822..843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcggcggcg gctgctgcaa aacagataca aaactacata agacccccac cttatatatt
       61 ctttcccacc cttaaccacg cccagatctt cgagtcgaca agcttgaatt cgatatctct
      121 agactcgagg cggccgcggt accagtatta cgcgctatga gtaacacaaa attattcaga
      181 tttcacttcc tcttattcag ttttcccgcg aaaatggcca aatcttactc ggttacgccc
      241 aaatttacta caacatccgc ctaaaaccgc gcgaaaattg tcacttcctg tgtacaccgg
      301 cgcacaccaa aaacgtcact tttgccacat ccgtcgctta catgtgttcc gccacacttg
      361 caacatcaca cttccgccac actactacgt cacccgcccc gttcccacgc cccgcgccac
      421 gtcacaaact ccaccccctc attatcatat tggcttcaat ccaaaataag gtatattatt
      481 gatgatgtta attaagaatt aattcgatcc tgaatggcga atggacgcgc cctgtagcgg
      541 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      601 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      661 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      721 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      781 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      841 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      901 ttcggcctat tggttaaaaa atgagctgat ttaacaaaa
//
