LOCUS       sequence-109803-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for use in AdEasy System. Used for expression of 
            transgenes under a chosen (non-CMV) promoter when no GFP tracer is 
            desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pShuttle
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   322..424
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      301 ggatccatgc atgttaatta acatcatcaa taatatacct tattttggat tgaagccaat
      361 atgataatga gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac
      421 gtaggtttta gggcggagta acttgtatgt gttgggaatt gtagttttct taaaatggga
      481 agtgacgtaa cgtgggaaaa cggaagtgac gatttgagga agttgtgggt tttttggctt
      541 tcgtttctgg gcgtaggttc gcgtgcggtt ttctgggtgt tttttgtgga ctttaaccgt
      601 tacgtcattt tttagtccta tatatactcg ctctgcactt ggcccttttt tacactgtga
      661 ctgattgagc tggtgccgtg tcgagtggtg tttttttaat aggttttctt ttttactggt
      721 aaggctgact gttatggctg ccgctgtgga agcgctgtat gttgttctgg agcgggaggg
      781 tgctattttg cctaggcagg agggtttttc aggtgtttat gtgtttttct ctcctattaa
      841 ttttgttata cctcctatgg gg
//
