LOCUS       sequence-109805-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pcDNA4-Vps34-Flag
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      246..255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tggtaccgag ctcggatcca ctagtccagt gtggtggaat tctgcagata tccagcacag
      241 tggcggccgc catgggggaa gcagagaagt ttcactacat ctatagttgt gacctggata
      301 tcaacgtcca gcttaagata ggaagcttgg aagggaagag agaacaaaag agttataaag
      361 ctgtcctgga agacccaatg ttgaagttct caggactata tcaagagaca tgctctgatc
      421 tttatgttac ttgtcaagtt tttgcagaag ggaagccttt ggccttgcca gtgagaacat
      481 cctacaaagc atttagtaca agatggaact ggaatgaatg gctgaaacta ccagtaaaat
      541 accctgacct gcccaggaat gcccaagtgg ccctcaccat atgggatgtg tatggtcccg
      601 gaaaagcagt gcctgtagga ggaacaacgg tttcgctctt tggaaaatac ggcatgtttc
      661 gccaagggat gcatgacttg aaagtctggc ctaatgtaga agcagatgga tcagaaccca
      721 caaaaactcc tggcagaaca agtagcactc tctcagaaga tcagatgagc cgtcttgcca
      781 agctcaccaa agctcatcga caaggacaca tggtgaaagt agattggctg gatagattga
      841 catttagaga aatagaaatg ataaatgaga gtgaaaaacg aagttctaat ttcatgtacc
      901 tgatggttga atttcgatgt gtcaagtgtg atg
//
