LOCUS       sequence-109812-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Yu J, Zhang L, Hwang PM, Rago C, Kinzler KW, Vogelstein B
  TITLE     Identification and classification of p53-regulated genes.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Dec 7. 96(25):14517-22.
  PUBMED    10588737
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Dec 7. 96(25):14517-22."
COMMENT     Sequence Label: tTA-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..126
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     154..173
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        154..172
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             189..809
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(262..281)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tcttcccttc tctgttcctc cgcagccagc catggatact ctgagtccaa accgggcccc
       61 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
      121 attgtgctgt ctcatcattt tggcaaagaa ttgtaatacg actcactata gggcgaatct
      181 agaccaccat gtcaagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
      241 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
      301 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
      361 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
      421 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
      481 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
      541 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
      601 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
      661 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
      721 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
      781 aaaaacaact taaatgtgaa agtgggtccg cgtacagccg cgcgcgtacg aaaaacaatt
      841 acgggtctac catcgagggc ctgctcgatc tcccggacga cgacgccccc gaagaggcgg
      901 ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca cacgc
//
