LOCUS       sequence-109821-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA targeting lnc-BATE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Alvarez-Dominguez JR, Bai Z, Xu D, Yuan B, Lo KA, Yoon MJ, Lim YC, 
            Knoll M, Slavov N, Chen S, Chen P, Lodish HF, Sun L
  TITLE     De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long
            Non-coding RNA Regulators of Brown Adipocyte Development.
  JOURNAL   Cell Metab. 2015 May 5;21(5):764-76. doi: 
            10.1016/j.cmet.2015.04.003. Epub 2015 Apr 23.
  PUBMED    25921091
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.04.003"; journalName: "Cell Metab"; date: 
            "2015-05-5- 5"; volume: "21"; issue: "5"; pages: "764-76"
COMMENT     Sequence Label: PMKO-sh_lnc-BATE1
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        77..325
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     77..97
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     248..267
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(410..430)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        415..744
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      595..730
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(753..769)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(754..770)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ccccccactc cttttcagcc ctcactcctt ctctaggcgc cggaattgaa gatctgtcga
       61 caaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
      121 caaggctgtt agagagataa ttagaattaa tttgactgca aacacaaaga tattagtaca
      181 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      241 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      301 tatcttgtgg aaaggacgaa acaccgggcc ttcctttaga cttgacctct cgagaggtca
      361 agtctaaagg aaggcttttt gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt
      421 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      481 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      541 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      601 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      661 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
      721 tttggaggcc taggcttttg caaaaagctt atcgataccg tcgacctcga gaccatggtg
      781 agcaagcaga tcctgaagaa caccggcctg caggagatca tgagcttcaa ggtgaacctg
      841 gagggcgtgg tgaacaacca cgtgttcacc atggagggct gcggcaaggg caacatcctg
      901 ttcggcaacc agctggtgca gatccgcgtg accaagggcg cccccctgcc cttcgccttc
      961 gacatcctga gccccgcctt ccagtacggc aaccgcacct tcaccaagta ccccgaggac
     1021 atcagcgact tcttcatcca gagcttcccc gccggcttcg tgtacgagcg caccctgcgc
     1081 tacaaggacg gcggcctggt ggagatccgc agcgac
//
