LOCUS       sequence-109823-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a scrambled shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Alvarez-Dominguez JR, Bai Z, Xu D, Yuan B, Lo KA, Yoon MJ, Lim YC, 
            Knoll M, Slavov N, Chen S, Chen P, Lodish HF, Sun L
  TITLE     De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long
            Non-coding RNA Regulators of Brown Adipocyte Development.
  JOURNAL   Cell Metab. 2015 May 5;21(5):764-76. doi: 
            10.1016/j.cmet.2015.04.003. Epub 2015 Apr 23.
  PUBMED    25921091
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.04.003"; journalName: "Cell Metab"; date: 
            "2015-05-5- 5"; volume: "21"; issue: "5"; pages: "764-76"
COMMENT     Sequence Label: PMKO-shNC
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        79..327
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     79..99
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     250..269
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(412..432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        417..746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      597..732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(755..771)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(756..772)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gccgctatct ctttatccag ccctcactcc ttctctaggc gccggaattg aagatctgtc
       61 gacaaggtcg ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga
      121 tacaaggctg ttagagagat aattagaatt aatttgactg caaacacaaa gatattagta
      181 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
      241 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
      301 tatatcttgt ggaaaggacg aaacaccggc aacaagatga agagcaccaa ctcgagttgg
      361 tgctcttcat cttgttgttt ttgaattcgc cagcacagtg gtcgaccctg tggaatgtgt
      421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
      541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
      601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
      661 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
      721 tttttggagg cctaggcttt tgcaaaaagc ttatcgatac cgtcgacctc gagaccatgg
      781 tgagcaagca gatcctgaag aacaccggcc tgcaggagat catgagcttc aaggtgaacc
      841 tggagggcgt ggtgaacaac cacgtgttca ccatggaggg ctgcggcaag ggcaacatcc
      901 tgttcggcaa ccagctggtg cagatccgcg tgaccaaggg cgcccccctg cccttcgcct
      961 tcgacatcct gagccccgcc ttccagtacg gcaaccgcac cttcaccaag taccccgagg
     1021 acatcagcga cttcttcatc caaagcttcc ccgccggctt cgtgtacgaa cgcaccctg
//
