LOCUS       sequence-109841-        1259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SaSrtA-KA
FEATURES             Location/Qualifiers
     source          1..1259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             447..1040
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             1071..1226
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             1242..1259
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgcaagctaa acctcaaatt ccgaaagata aatcgaaagt ggcaggctat attgaaattc
       61 cagatgctga tattaaagaa ccagtatatc caggaccagc aacacctgaa caattaaata
      121 gaggtgtaag ctttgcaaag gaaaatgcct cactagatga tcaaaatatt tcaattgcag
      181 gacacacttt cattgaccgt ccgaactatc aatttacaaa tcttaaagca gccaaaaaag
      241 gtagtatggt gtactttaaa gttggtaatg aaacacgtaa gtataaaatg acaagtataa
      301 gagatgttaa gcctacagat gtagaagttc tagatgaaca aaaaggtaaa gataaacaat
      361 taacattaat tacttgtgat gattacaatg aaaagacagg cgtttgggaa aaacgtaaaa
      421 tctttgtagc tacagaagtc aaaggctgca tcacgggaga tgcactagtt gccctacccg
      481 agggcgagtc ggtacgcatc gccgacatcg tgccgggtgc gcggcccaac agtgacaacg
      541 ccatcgacct gaaagtcctt gaccggcatg gcaatcccgt gctcgccgac cggctgttcc
      601 actccggcga gcatccggtg tacacggtgc gtacggtcga aggtctgcgt gtgacgggca
      661 ccgcgaacca cccgttgttg tgtttggtcg acgtcgccgg ggtgccgacc ctgctgtgga
      721 agctgatcga cgaaatcaag ccgggcgatt acgcggtgat tcaacgcagc gcattcagcg
      781 tcgactgtgc aggttttgcc cgcgggaaac ccgaatttgc gcccacaacc tacacagtcg
      841 gcgtccctgg actggtgcgt ttcttggaag cacaccaccg agacccggac gcccaagcta
      901 tcgccgacga gctgaccgac gggcggttct actacgcgaa agtcgccagt gtcaccgacg
      961 ccggcgtgca gccggtgtat agccttcgtg tcgacacggc agaccacgcg tttatcacga
     1021 acgggttcgt cagccacgct actggcctca ccggtctgaa ctcaggcctc acgacaaatc
     1081 ctggtgtatc cgcttggcag gtcaacacag cttatactgc gggacaattg gtcacatata
     1141 acggcaagac gtataaatgt ttgcagcccc acacctcctt ggcaggatgg gaaccatcca
     1201 acgttcctgc cttgtggcag cttcaagcgg ccgcactcga gcaccaccac caccaccac
//
