LOCUS       sequence-109844-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SaSrtA-KQ
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             448..1041
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             1072..1227
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             1243..1260
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaagcta aacctcaaat tccgaaagat aaatcgaaag tggcaggcta tattgaaatt
       61 ccagatgctg atattaaaga accagtatat ccaggaccag caacacctga acaattaaat
      121 agaggtgtaa gctttgcaaa ggaaaatcag tcactagatg atcaaaatat ttcaattgca
      181 ggacacactt tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa
      241 ggtagtatgg tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata
      301 agagatgtta agcctacaga tgtagaagtt ctagatgaac aaaaaggtaa agataaacaa
      361 ttaacattaa ttacttgtga tgattacaat gaaaagacag gcgtttggga aaaacgtaaa
      421 atctttgtag ctacagaagt caaaggctgc atcacgggag atgcactagt tgccctaccc
      481 gagggcgagt cggtacgcat cgccgacatc gtgccgggtg cgcggcccaa cagtgacaac
      541 gccatcgacc tgaaagtcct tgaccggcat ggcaatcccg tgctcgccga ccggctgttc
      601 cactccggcg agcatccggt gtacacggtg cgtacggtcg aaggtctgcg tgtgacgggc
      661 accgcgaacc acccgttgtt gtgtttggtc gacgtcgccg gggtgccgac cctgctgtgg
      721 aagctgatcg acgaaatcaa gccgggcgat tacgcggtga ttcaacgcag cgcattcagc
      781 gtcgactgtg caggttttgc ccgcgggaaa cccgaatttg cgcccacaac ctacacagtc
      841 ggcgtccctg gactggtgcg tttcttggaa gcacaccacc gagacccgga cgcccaagct
      901 atcgccgacg agctgaccga cgggcggttc tactacgcga aagtcgccag tgtcaccgac
      961 gccggcgtgc agccggtgta tagccttcgt gtcgacacgg cagaccacgc gtttatcacg
     1021 aacgggttcg tcagccacgc tactggcctc accggtctga actcaggcct cacgacaaat
     1081 cctggtgtat ccgcttggca ggtcaacaca gcttatactg cgggacaatt ggtcacatat
     1141 aacggcaaga cgtataaatg tttgcagccc cacacctcct tggcaggatg ggaaccatcc
     1201 aacgttcctg ccttgtggca gcttcaagcg gccgcactcg agcaccacca ccaccaccac
//
