LOCUS       sequence-109845-         447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SaSrtA-KQ
FEATURES             Location/Qualifiers
     source          1..447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcaagcta aacctcaaat tccgaaagat aaatcgaaag tggcaggcta tattgaaatt
       61 ccagatgctg atattaaaga accagtatat ccaggaccag caacacctga acaattaaat
      121 agaggtgtaa gctttgcaaa ggaaaatcag tcactagatg atcaaaatat ttcaattgca
      181 ggacacactt tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa
      241 ggtagtatgg tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata
      301 agagatgtta agcctacaga tgtagaagtt ctagatgaac aaaaaggtaa agataaacaa
      361 ttaacattaa ttacttgtga tgattacaat gaaaagacag gcgtttggga aaaacgtaaa
      421 atctttgtag ctacagaagt caaaggc
//
