LOCUS       sequence-109848-        1326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the wild type of Streptococcus pyogenes sortase A in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SpSrtA
FEATURES             Location/Qualifiers
     source          1..1326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             514..1107
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             1138..1293
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             1309..1326
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtcggtcc tgcaagctca aatggccgct caacaactgc cggtcatcgg tggtatcgct
       61 atcccggaac tgggtatcaa cctgccgatt tttaaaggcc tgggtaacac cgaactgatc
      121 tatggcgccg gtacgatgaa agaagaacag gtgatgggcg gtgaaaacaa ttacagtctg
      181 gcttcccatc acatttttgg catcaccggt agctctcaga tgctgttcag cccgctggaa
      241 cgtgcgcaaa atggcatgag catctacctg accgataaag aaaaaatcta cgaatacatc
      301 atcaaagatg tctttacggt ggcgccggaa cgtgtggacg ttattgatga caccgccggc
      361 ctgaaagaag ttacgctggt cacctgcacg gatatcgaag caaccgaacg cattatcgtt
      421 aaaggtgaac tgaaaacgga atacgacttc gacaaagcac cggcggatgt tctgaaagca
      481 tttaatcatt cttacaacca ggtctccacg ggctgcatca cgggagatgc actagttgcc
      541 ctacccgagg gcgagtcggt acgcatcgcc gacatcgtgc cgggtgcgcg gcccaacagt
      601 gacaacgcca tcgacctgaa agtccttgac cggcatggca atcccgtgct cgccgaccgg
      661 ctgttccact ccggcgagca tccggtgtac acggtgcgta cggtcgaagg tctgcgtgtg
      721 acgggcaccg cgaaccaccc gttgttgtgt ttggtcgacg tcgccggggt gccgaccctg
      781 ctgtggaagc tgatcgacga aatcaagccg ggcgattacg cggtgattca acgcagcgca
      841 ttcagcgtcg actgtgcagg ttttgcccgc gggaaacccg aatttgcgcc cacaacctac
      901 acagtcggcg tccctggact ggtgcgtttc ttggaagcac accaccgaga cccggacgcc
      961 caagctatcg ccgacgagct gaccgacggg cggttctact acgcgaaagt cgccagtgtc
     1021 accgacgccg gcgtgcagcc ggtgtatagc cttcgtgtcg acacggcaga ccacgcgttt
     1081 atcacgaacg ggttcgtcag ccacgctact ggcctcaccg gtctgaactc aggcctcacg
     1141 acaaatcctg gtgtatccgc ttggcaggtc aacacagctt atactgcggg acaattggtc
     1201 acatataacg gcaagacgta taaatgtttg cagccccaca cctccttggc aggatgggaa
     1261 ccatccaacg ttcctgcctt gtggcagctt caagcggccg cactcgagca ccaccaccac
     1321 caccac
//
