LOCUS       sequence-109854-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human Parkin fused to eYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Tait SW, Oberst A, Quarato G, Milasta S, Haller M, Wang R, Karvela 
            M, Ichim G, Yatim N, Albert ML, Kidd G, Wakefield R, Frase S, 
            Krautwald S, Linkermann A, Green DR
  TITLE     Widespread mitochondrial depletion via mitophagy does not compromise
            necroptosis.
  JOURNAL   Cell Rep. 2013 Nov 27;5(4):878-85. doi: 
            10.1016/j.celrep.2013.10.034. Epub 2013 Nov 21.
  PUBMED    24268776
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.10.034"; journalName: "Cell Rep"; date: 
            "2013-11-27- 27"; volume: "5"; issue: "4"; pages: "878-85"
COMMENT     Sequence Label: YFP-Parkin-IRES-zeo
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(20..22,23..25,26..736)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(65..86)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(326..345)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     673..694
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atctaagtaa gtaggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
       61 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      121 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      181 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg
      241 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      301 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      361 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      421 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      481 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      541 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      601 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
      661 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gctttgatag tgtttgtcag
      781 gttcaactcc agccatggtt tcccagtgga ggtcgattct gacaccagca tcttccagct
      841 caaggaggtg
//
