LOCUS       sequence-109857-       10064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Gutscher M, Sobotta MC, Wabnitz GH, Ballikaya S, Meyer AJ, Samstag 
            Y, Dick TP
  TITLE     Proximity-based protein thiol oxidation by H2O2-scavenging 
            peroxidases.
  JOURNAL   J Biol Chem. 2009 Nov 13;284(46):31532-40. doi: 
            10.1074/jbc.M109.059246. Epub 2009 Sep 15.
  PUBMED    19755417
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.059246"; journalName: "J Biol Chem"; date: 
            "2009-11-13- 13"; volume: "284"; issue: "46"; pages: "31532-40"
COMMENT     Sequence Label: pEIGW roGFP2-Orp1
FEATURES             Location/Qualifiers
     source          1..10064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1304..1537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2033..2150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2207..2418
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2465..2474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2471..2473,2474..2476,2477..2479,2480..3190)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MAVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(2519..2540)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2780..2799)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3127..3148
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3839..3855)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3840..3856)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    3869..4457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3922..3942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4340..4351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4626..4659)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4679..4859
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4884..4902)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4885..4902)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5192..5211)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5425..5444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5544..5566
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5604..5622)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5690..5794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5795..5863,5864..6655)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6013..6032)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6826..7414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7315..7334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7568..7585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7655..7675)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7660..7989
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7840..7975
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9123..9188
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9318..9338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9763..9897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9800..9819)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9854..9873
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     2041 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2101 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
     2161 agactagcct cgagaagctt gatcgatggc tccggtgccc gtcagtgggc agagcgcaca
     2221 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     2281 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     2341 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     2401 tttgccgcca gaacacaggt gtcgtgacgc ggatcgggat cggcctttac ggccggttac
     2461 ccgggccacc atggcagtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2521 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2581 cgatgccacc tacggcaagc tgaccctgaa gttcatctcc accaccggca agctgcccgt
     2641 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     2701 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2761 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2821 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2881 catcctgggg cacaagctgg agtacaacta caactgccac aacgtctata tcatggccga
     2941 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3001 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3061 gcccgacaac cactacctga gcacctgctc cgccctgagc aaagacccca acgagaagcg
     3121 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3181 gctgtacaag actagtggtg gttcaggtgg tggtggttca ggtggtggtg gttcaggtgg
     3241 aggaggatca ggaggaggag gatcaggagg aggaggatca ggaggagaat tcgatatctc
     3301 agaattctat aagctagcac ctgttgacaa gaaaggccaa ccattcccct tcgaccaatt
     3361 aaagggaaaa gtggtgctta tcgttaatgt tgcctccaaa tgtggattca ctcctcaata
     3421 caaagaacta gaggccttgt acaaacgtta taaggacgaa ggatttacca tcatcgggtt
     3481 cccatgcaac cagtttggcc accaagaacc tggctctgat gaagaaattg cccagttctg
     3541 ccaactgaac tatggcgtga ctttccccat tatgaaaaaa attgacgtta atggtggcaa
     3601 tgaggaccct gtttacaagt ttttgaagag ccaaaaatcc ggtatgttgg gcttgagagg
     3661 tatcaaatgg aattttgaaa aattcttagt cgataaaaag ggtaaagtgt acgaaagata
     3721 ctcttcacta accaaacctt cttcgttgtc cgaaaccatc gaagaacttt tgaaagaggt
     3781 ggaaggccgc gactctagct agtcgagctc aacttcgaat tcgatatcaa gcttatcgcg
     3841 ataccgtcga cctcgaggga attccgataa tcaacctctg gattacaaaa tttgtgaaag
     3901 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3961 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4021 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4081 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4141 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4201 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4261 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4321 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4381 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4441 ttgggccgcc tccccgcatc gggaattcga gctcggtcga ggctagtctt gcagagctcg
     4501 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4561 aaggggggac tggaagggct aattcactcc caacgaagac aagatgggat caattcacca
     4621 tgggaataac ttcgtatagc atacattata cgaagttatg ctgctttttg cttgtactgg
     4681 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4741 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4801 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4861 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     4921 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     4981 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     5041 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     5101 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     5161 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     5221 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     5281 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     5341 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     5401 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5461 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5521 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5581 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5641 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5701 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5761 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5821 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5881 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5941 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6001 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6061 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6121 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6181 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6241 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6301 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6361 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6421 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6481 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6541 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6601 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6661 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6721 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6781 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6841 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6901 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6961 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7021 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7081 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7141 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7201 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7261 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7321 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7381 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7441 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7501 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7561 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7621 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     7681 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7741 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7801 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7861 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7921 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7981 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     8041 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     8101 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     8161 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     8221 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     8281 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     8341 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     8401 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     8461 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     8521 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     8581 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     8641 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     8701 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     8761 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     8821 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     8881 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     8941 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     9001 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     9061 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     9121 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     9181 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     9241 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     9301 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     9361 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     9421 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     9481 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     9541 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     9601 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     9661 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     9721 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     9781 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9841 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     9901 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     9961 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    10021 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtag
//
