LOCUS       sequence-109858-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of cas9 and inducible expression of lambda 
            RED and sgR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S
  TITLE     Multigene editing in the Escherichia coli genome using the 
            CRISPR-Cas9 system.
  JOURNAL   Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
  PUBMED    25636838
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14."
COMMENT     Sequence Label: pCas
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(5..83)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
       61 gctatgctgt tttgaatggt tccaacaaga ttattttata acttttataa caaataatca
      121 aggagaaatt caaagaaatt tatcagccat aaaacaatac ttaatactat agaatgataa
      181 caaaataaac tactttttaa aagaattttg tgttataatc tatttattat taagtattgg
      241 gtaatatttt ttgaagagat attttgaaaa agaaaaatta aagcatatta aactaatttc
      301 ggaggtcatt aaaactatta ttgaaatcat caaactcatt atggatttaa tttaaacttt
      361 ttattttagg aggcaaaaat ggataagaaa tactcaatag gcttagatat cggcacaaat
      421 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt
      481 ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac
      541 agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt
      601 cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat
      661 gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa
      721 cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact
      781 atctatcatc tgcgaaaaa
//
