LOCUS       sequence-109859-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of cas9 and inducible expression of lambda 
            RED and sgR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S
  TITLE     Multigene editing in the Escherichia coli genome using the 
            CRISPR-Cas9 system.
  JOURNAL   Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
  PUBMED    25636838
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14."
COMMENT     Sequence Label: pCas
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
       61 gtctagatta agaaataatc ttcatctaaa atatacttca gtcacctcct agctgactca
      121 aatcaatgcg tgtttcataa agaccagtga tggattgatg gataagagtg gcatctaaaa
      181 cttcttttgt agacgtatat cgtttacgat caattgttgt atcaaaatat ttaaaagcag
      241 cgggagctcc aagattcgtc aacgtaaata aatgaataat attttctgct tgttcacgta
      301 ttggtttgtc tctatgtttg ttatatgcac taagaacttt atctaaattg gcatctgcta
      361 aaataacacg cttagaaaat tcactgattt gctcaataat ctcatctaaa taatgcttat
      421 gctgctccac aaacaattgt ttttgttcgt tatcttctgg actacccttc aacttttcat
      481 aatgactagc taaatataaa aaattcacat atttgcttgg cagagccagc tcatttcctt
      541 tttgtaattc tccggcacta gccagcatcc gtttacgacc gttttctaac tcaaaaagac
      601 tatatttagg tagtttaatg attaagtctt ttttaacttc cttatatcct ttagcttcta
      661 aaaagtcaat cggatttttt tcaaaggaac ttctttccat aattgtgatc cctagtaact
      721 ctttaacgga ttttaacttc ttcgatttcc ctttttccac cttagcaacc actaggactg
      781 aataagctac cgttggacta tcaaaaccac catatttttt
//
