LOCUS       sequence-109862-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For protein expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Tabata K, Matsunaga K, Sakane A, Sasaki T, Noda T, Yoshimori T
  TITLE     Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic 
            pathway via a novel Rab7-binding domain.
  JOURNAL   Mol Biol Cell. 2010 Dec;21(23):4162-72. doi: 
            10.1091/mbc.E10-06-0495. Epub 2010 Oct 13.
  PUBMED    20943950
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E10-06-0495"; journalName: "Mol Biol Cell"; date: 
            "2010-12"; volume: "21"; issue: "23"; pages: "4162-72"
COMMENT     Sequence Label: pCold-TF-hRubicon
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aagtgcatca tcatcatcat cacatgcaag tttcagttga aaccactcaa ggccttggcc
       61 gccgtgtaac gattactatc gctgctgaca gcatcgagac cgctgttaaa agcgagctgg
      121 tcaacgttgc gaaaaaagta cgtattgacg gcttccgcaa gggcaaagtg ccaatgaata
      181 tcgttgctca gcgttatggc gcgtctgtac gccaggacgt tctgggtgac ctgatgagcc
      241 gtaacttcat tgacgccatc attaaagaaa aaatcaatcc ggctggcgca ccgacttatg
      301 ttccgggcga atacaagctg ggtgaagact tcacttactc tgtagagttt gaagtttatc
      361 cggaagttga actgcaaggt ctggaagcga tcgaagttga aaaaccgatc gttgaagtga
      421 ccgacgctga cgttgacggc atgctggata ctctgcgtaa acagcaggcg acctggaaag
      481 aaaaagacgg cgctgttgaa gcagaagacc gcgtgaccat cgacttcacc ggttctgtag
      541 acggcgaaga gttcgaaggc ggtaaagcgt ctgatttcgt actggcgatg ggccagggtc
      601 gtatgatccc gggctttgaa gacggtatca aaggccacaa agctggcgaa gagttcacca
      661 tcgacgtgac cttcccggaa gaataccacg cagaaaacct gaaaggtaaa gcagcgaaat
      721 tcgctatcaa cctgaagaaa gttgaagagc gtgaactgcc ggaactgacc gcagagttca
      781 tcaaacgttt cggcgttgaa gatggttccg tagaaggtct gcgcgctgaa gtgcgtaaaa
      841 acatggagcg cgagctgaag agcgccatcc gtaaccgcgt taagtctcag gcgatcgaag
      901 gtctggtaa
//
