LOCUS       sequence-109863-       10449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression
            system in drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 10449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pUAST mito roGFP2-Orp1
FEATURES             Location/Qualifiers
     source          1..10449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             27..101
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(126..128,129..839)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(168..189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(429..448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     776..797
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          1520..1585
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1715..1735
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2160..2294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2197..2216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2251..2270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2321..2339)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2799..2870,3265..3538,3613..4267,4329..4644,4865..4996,
                     5067..5681)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(6459..7013,7014..7044)
                     /label=P element 5' end
     rep_origin      complement(7279..7867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7359..7378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8038..8829,8830..8898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8661..8680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8899..9003)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9071..9089
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9127..9149)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9249..9268
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9805..9835,9836..10037)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    10085..10179
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        10198..10436
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     10383..10404
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gaattcgtta acagatctgc ggccgcatgt ccgtcctgac gccgctgctg ctgcggggct
       61 tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg ttgccgccgg
      121 gatccgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      241 acggcaagct gaccctgaag ttcatctcca ccaccggcaa gctgcccgtg ccctggccca
      301 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      541 acaagctgga gtacaactac aactgccaca acgtctatat catggccgac aagcagaaga
      601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      721 actacctgag cacctgctcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
      781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
      841 ctagtggtgg ttcaggtggt ggtggttcag gtggtggtgg ttcaggtgga ggaggatcag
      901 gaggaggagg atcaggagga ggaggatcag gaggagaatt cgatatctca gaattctata
      961 agctagcacc tgttgacaag aaaggccaac cattcccctt cgaccaatta aagggaaaag
     1021 tggtgcttat cgttaatgtt gcctccaaat gtggattcac tcctcaatac aaagaactag
     1081 aggccttgta caaacgttat aaggacgaag gatttaccat catcgggttc ccatgcaacc
     1141 agtttggcca ccaagaacct ggctctgatg aagaaattgc ccagttctgc caactgaact
     1201 atggcgtgac tttccccatt atgaaaaaaa ttgacgttaa tggtggcaat gaggaccctg
     1261 tttacaagtt tttgaagagc caaaaatccg gtatgttggg cttgagaggt atcaaatgga
     1321 attttgaaaa attcttagtc gataaaaagg gtaaagtgta cgaaagatac tcttcactaa
     1381 ccaaaccttc ttcgttgtcc gaaaccatcg aagaactttt gaaagaggtg gaatagtcta
     1441 gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     1501 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     1561 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     1621 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     1681 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     1741 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     1801 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     1861 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     1921 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     1981 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     2041 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2101 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2161 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     2221 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     2281 atcatgtctg gatcggatcc actagaaggc cttagtatgt atgtaagtta ataaaaccct
     2341 tttttggaga atgtagattt aaaaaaacat attttttttt tattttttac tgcactggac
     2401 atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat ttgaagtacc
     2461 aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag cgctgtttgc
     2521 ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt
     2581 cgccaccgtt tgtagcgtta cctagcgtca atgtccgcct tcagttgcac tttgtcagcg
     2641 gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa
     2701 aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt gattggtttt
     2761 gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc
     2821 tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat gtgagtagta
     2881 catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa catcaaattg
     2941 tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaatcgcac aaacaaatag
     3001 tgacacgaaa cagattattc tggtagctgt gctcgctata taagacaatt tttaagatca
     3061 tatcatgatc aagacatcta aaggcattca ttttcgacta cattcttttt tacaaaaaat
     3121 ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa aagtataaac
     3181 ctacttcgta ggatacttcg ttttgttcgg ggttagatga gcataacgct tgtagttgat
     3241 atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac
     3301 cagggcttcg ggcaggccaa aaactacggc acgctcctgc cacccagtcc gccggaggac
     3361 tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca caatatggac
     3421 atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc
     3481 ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt
     3541 gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa
     3601 tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt
     3661 ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc
     3721 aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc
     3781 aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg
     3841 aacacctgat tttccaggcc atggtgcgga tgccacgaca tctgacctat cggcagcgag
     3901 tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca
     3961 tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg
     4021 cctccgaggc actaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg
     4081 actcatttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga
     4141 ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt gacaagatcc
     4201 ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct
     4261 tttcctagtg agttcgatgt gtttattaag ggtatctagc attacattac atctcaactc
     4321 ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag
     4381 gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc
     4441 gacaattttg ctattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat
     4501 ttggagaagc cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg
     4561 cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta
     4621 aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct
     4681 tacaattctt ggaaacaaat tcgctagatt ttagttagaa ttgcctgatt ccacaccctt
     4741 cttagttttt ttcaatgaga tgtatagttt atagttttgc agaaaataaa taaatttcat
     4801 ttaactcgcg aacatgttga agatatgaat attaatgaga tgcgagtaac attttaattt
     4861 gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg
     4921 cgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt
     4981 ctttgccacg ataaatgtaa gtcttgttta gaatacattt gcatattaat aatttactaa
     5041 ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg
     5101 aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat
     5161 taccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac
     5221 tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg
     5281 tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt
     5341 ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg
     5401 gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg
     5461 agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt
     5521 cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg
     5581 ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg
     5641 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
     5701 actgcttgtt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa
     5761 aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt
     5821 atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt
     5881 acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata
     5941 ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc
     6001 acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt
     6061 gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt
     6121 ccgagtggtt catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat
     6181 ttgctccaat atatttgtat atatttccct cacagctata tttattctaa tttaatatta
     6241 tgacttttta aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact
     6301 gaaagtgaca tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata
     6361 atagtgttgt ttatatatat caaaaataac aactataata ataagaatac atttaattta
     6421 gaaaatgctt ggatttcact ggaactagaa ttaattcggc tgctgctcta aacgacgcat
     6481 ttcgtactcc aaagtacgaa ttttttccct caagctctta ttttcattaa acaatgaaca
     6541 ggacctaacg cacagtcacg ttattgttta cataaatgat tttttttact attcaaactt
     6601 actctgtttg tgtactccca ctggtatagc cttcttttat cttttctggt tcaggctcta
     6661 tcactttact aggtacggca tctgcgttga gtcgcctcct tttaaatgtc tgaccttttg
     6721 caggtgcagc cttccactgc gaatcattaa agtgggtatc acaaatttgg gagttttcac
     6781 caaggctgca cccaaggctc tgctcccaca attttctctt aatagcacac ttcggcacgt
     6841 gaattaattt tactccagtc acagctttgc agcaaaattt gcaatatttc attttttttt
     6901 attccacgta agggttaatg ttttcaaaaa aaaattcgtc cgcacacaac ctttcctctc
     6961 aacaagcaaa cgtgcactga atttaagtgt atacttcggt aagcttcggc tatcgacggg
     7021 accaccttat gttatttcat catgggccag acccacgtag tccagcggca gatcggcggc
     7081 ggagaagtta agcgtctcca ggatgacctt gcccgaactg gggcacgtgg tgttcgacga
     7141 tgtgcagcta atttcgcccg gctccacgtc cgcccattgg ttaatcagca gaccctcgtt
     7201 ggcgtaacgg aaccatgaga ggtacgacaa ccatttgagg tatactggca ccgagcccga
     7261 gttcaagaag aaggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7441 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     7501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7921 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7981 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8041 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8101 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8161 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8221 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8281 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8341 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8401 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8461 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8521 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8581 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8641 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8701 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8761 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8821 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8881 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8941 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9001 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9061 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     9121 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     9181 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     9241 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     9301 gcaccgaatc gcgcggaact aacgacagtc gctccaaggt cgtcgaacaa aaggtgaatg
     9361 tgttgcggag agcgggtggg agacagcgaa agagcaacta cgaaacgtgg tgtggtggag
     9421 gtgaattatg aagagggcgc gcgatttgaa aagtatgtat ataaaaaata tatcccggtg
     9481 ttttatgtag cgataaacga gtttttgatg taaggtatgc aggtgtgtaa gtcttttggt
     9541 tagaagacaa atccaaagtc tacttgtggg gatgttcgaa ggggaaatac ttgtattcta
     9601 taggtcatat cttgttttta ttggcacaaa tataattaca ttagcttttt gagggggcaa
     9661 taaacagtaa acacgatggt aataatggta aaaaaaaaaa caagcagtta tttcggatat
     9721 atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga
     9781 agctcacgat gagaatggcc agaccatgat gaaataacat aaggtggtcc cgtcggcaag
     9841 agacatccac ttaacgtatg cttgcaataa gtgcgagtga aaggaatagt attctgagtg
     9901 tcgtattgag tctgagtgag acagcgatat gattgttgat taacccttag catgtccgtg
     9961 gggtttgaat taactcataa tattaattag acgaaattat ttttaaagtt ttatttttaa
    10021 taatttgcga gtacgcaaag cttgggctgc atctctccgg atccaagctt gcatgcctgc
    10081 aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
    10141 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta gcgagcgccg
    10201 gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga
    10261 acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc
    10321 tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa
    10381 ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata
    10441 gggaattgg
//
