LOCUS       sequence-109865-       10267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for expression of mitochondrial roGFP2-Grx1 with the Gal4 expression
            system in drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 10267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pUAST mito roGFP2-Grx1
FEATURES             Location/Qualifiers
     source          1..10267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..81
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(106..108,109..819)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(148..169)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(409..428)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     756..777
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          1326..1391
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1966..2100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2003..2022)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2057..2076
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2127..2145)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2605..2676,3071..3344,3419..4073,4135..4450,4671..4802,
                     4873..5487)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(6265..6819,6820..6850)
                     /label=P element 5' end
     rep_origin      complement(7085..7673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7165..7184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7844..8635,8636..8704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8467..8486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8705..8809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8877..8895
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8933..8955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9055..9074
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9611..9641,9642..9843)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    9891..9985
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        10004..10242
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     10189..10210
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 agatctatgt ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg
       61 ctcccagtgc cgcgcgccaa gatccattcg ttgccgccgg gatccgtgag caagggcgag
      121 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      181 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      241 ttcatctcca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      301 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      361 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      421 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      481 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      541 aactgccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      601 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      661 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacctgctcc
      721 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      781 gccgccggga tcactctcgg catggacgag ctgtacaaga ctagtggtgg ttcaggtggt
      841 ggtggttcag gtggtggtgg ttcaggtgga ggaggatcag gaggaggagg atcaggagga
      901 ggaggatcag gaggagaatt cgctcaagag tttgtgaact gcaaaatcca gcctgggaag
      961 gtggttgtgt tcatcaagcc cacctgcccg tactgcagga gggcccaaga gatcctcagt
     1021 caattgccca tcaaacaagg gcttctggaa tttgtcgata tcacagccac caaccacact
     1081 aacgagattc aagattattt gcaacagctc acgggagcaa gaacggtgcc tcgagtcttt
     1141 attggtaaag attgtatagg cggatgcagt gatctagtct ctttgcaaca gagtggggaa
     1201 ctgctgacgc ggctaaagca gattggagct ctgcagggta cctctagagg atctttgtga
     1261 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     1321 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     1381 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     1441 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     1501 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     1561 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     1621 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     1681 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     1741 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     1801 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     1861 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1921 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1981 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2041 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     2101 ggatccacta gaaggcctta gtatgtatgt aagttaataa aacccttttt tggagaatgt
     2161 agatttaaaa aaacatattt tttttttatt ttttactgca ctggacatca ttgaacttat
     2221 ctgatcagtt ttaaatttac ttcgatccaa gggtatttga agtaccaggt tctttcgatt
     2281 acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc
     2341 acagaaatat cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc accgtttgta
     2401 gcgttaccta gcgtcaatgt ccgccttcag ttgcactttg tcagcggttt cgtgacgaag
     2461 ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt
     2521 cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag
     2581 gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga
     2641 ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc
     2701 ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga
     2761 actgcgaccc acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac acgaaacaga
     2821 ttattctggt agctgtgctc gctatataag acaattttta agatcatatc atgatcaaga
     2881 catctaaagg cattcatttt cgactacatt cttttttaca aaaaatataa caaccagata
     2941 ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat
     3001 acttcgtttt gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct
     3061 atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca
     3121 ggccaaaaac tacggcacgc tcctgccacc cagtccgccg gaggactccg gttcagggag
     3181 cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt
     3241 caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa
     3301 cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc
     3361 agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt
     3421 tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag
     3481 acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc
     3541 gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc
     3601 tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc
     3661 caggccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat
     3721 caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc
     3781 agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggcacta
     3841 accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc atttaccgcc
     3901 cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc
     3961 attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag
     4021 ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt
     4081 cgatgtgttt attaagggta tctagcatta cattacatct caactcctat ccagcgtggg
     4141 tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt
     4201 gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgctat
     4261 tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatttgg agaagccact
     4321 ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt
     4381 cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat
     4441 tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa
     4501 acaaattcgc tagattttag ttagaattgc ctgattccac acccttctta gtttttttca
     4561 atgagatgta tagtttatag ttttgcagaa aataaataaa tttcatttaa ctcgcgaaca
     4621 tgttgaagat atgaatatta atgagatgcg agtaacattt taatttgcag atggttgcca
     4681 tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggcgtg atgaatatca
     4741 acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt gccacgataa
     4801 atgtaagtct tgtttagaat acatttgcat attaataatt tactaacttt ctaatgaatc
     4861 gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc ccgaagtcga
     4921 ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattacc gctttttctc
     4981 acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg ggccggagtg
     5041 ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc
     5101 ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt gggtccgccg
     5161 gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc ggtgccagta
     5221 tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt
     5281 aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc
     5341 cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg
     5401 gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta
     5461 agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt
     5521 tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat
     5581 tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa
     5641 catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac
     5701 cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat
     5761 acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg
     5821 ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg
     5881 gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt
     5941 tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat
     6001 ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt
     6061 aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca
     6121 gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat
     6181 atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa atgcttggat
     6241 ttcactggaa ctagaattaa ttcggctgct gctctaaacg acgcatttcg tactccaaag
     6301 tacgaatttt ttccctcaag ctcttatttt cattaaacaa tgaacaggac ctaacgcaca
     6361 gtcacgttat tgtttacata aatgattttt tttactattc aaacttactc tgtttgtgta
     6421 ctcccactgg tatagccttc ttttatcttt tctggttcag gctctatcac tttactaggt
     6481 acggcatctg cgttgagtcg cctcctttta aatgtctgac cttttgcagg tgcagccttc
     6541 cactgcgaat cattaaagtg ggtatcacaa atttgggagt tttcaccaag gctgcaccca
     6601 aggctctgct cccacaattt tctcttaata gcacacttcg gcacgtgaat taattttact
     6661 ccagtcacag ctttgcagca aaatttgcaa tatttcattt ttttttattc cacgtaaggg
     6721 ttaatgtttt caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg
     6781 cactgaattt aagtgtatac ttcggtaagc ttcggctatc gacgggacca ccttatgtta
     6841 tttcatcatg ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg
     6901 tctccaggat gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt
     6961 cgcccggctc cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc
     7021 atgagaggta cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg
     7081 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7261 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     7321 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7381 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7441 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7501 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     7561 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7621 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7681 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7741 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7801 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7861 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7921 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7981 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8041 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8101 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8161 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8221 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8281 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8341 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8401 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8461 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8521 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8581 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8641 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8701 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8761 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8821 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     8881 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     8941 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9001 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     9061 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc
     9121 ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg
     9181 ggtgggagac agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga
     9241 gggcgcgcga tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat
     9301 aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc
     9361 aaagtctact tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg
     9421 tttttattgg cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac
     9481 gatggtaata atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc
     9541 ttgcgtcggg cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga
     9601 atggccagac catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa
     9661 cgtatgcttg caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg
     9721 agtgagacag cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac
     9781 tcataatatt aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac
     9841 gcaaagcttg ggctgcatct ctccggatcc aagcttgcat gcctgcaggt cggagtactg
     9901 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     9961 tccgagcgga gtactgtcct ccgagcggag actctagcga gcgccggagt ataaatagag
    10021 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
    10081 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gtaaagtgca
    10141 agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc aactactgaa
    10201 atctgccaag aagtaattat tgaatacaag aagagaactc tgaataggga attgggaatt
    10261 cgttaac
//
