LOCUS       sequence-109866-       10174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for expression of cytosolic Grx1-roGFP2 with the Gal4 expression 
            system in drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pUAST cyto Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..10174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(439..441,442..1155)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(481..502)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(742..761)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1089..1110
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          1245..1310
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1440..1460
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1885..2019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1922..1941)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1976..1995
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2046..2064)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2524..2595,2990..3263,3338..3992,4054..4369,4590..4721,
                     4792..5406)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(6184..6738,6739..6769)
                     /label=P element 5' end
     rep_origin      complement(7004..7592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7084..7103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7763..8554,8555..8623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8386..8405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8624..8728)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8796..8814
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8852..8874)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8974..8993
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9530..9560,9561..9762)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    9810..9904
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        9923..10161
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     10108..10129
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gaattcgtta acagatctat ggctcaagag tttgtgaact gcaaaatcca gcctgggaag
       61 gtggttgtgt tcatcaagcc cacctgcccg tactgcagga gggcccaaga gatcctcagt
      121 caattgccca tcaaacaagg gcttctggaa tttgtcgata tcacagccac caaccacact
      181 aacgagattc aagattattt gcaacagctc acgggagcaa gaacggtgcc tcgagtcttt
      241 attggtaaag attgtatagg cggatgcagt gatctagtct ctttgcaaca gagtggggaa
      301 ctgctgacgc ggctaaagca gattggagct ctgcagacta gtggtggttc aggtggtggt
      361 ggttcaggtg gtggtggttc aggtggagga ggatcaggag gaggaggatc aggaggagga
      421 ggatcaggag gagaattcgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      481 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      541 ggcgatgcca cctacggcaa gctgaccctg aagttcatct ccaccaccgg caagctgccc
      601 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      661 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      721 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      781 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      841 aacatcctgg ggcacaagct ggagtacaac tacaactgcc acaacgtcta tatcatggcc
      901 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      961 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1021 ctgcccgaca accactacct gagcacctgc tccgccctga gcaaagaccc caacgagaag
     1081 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1141 gagctgtaca agtaaggtac ctctagagga tctttgtgaa ggaaccttac ttctgtggtg
     1201 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     1261 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     1321 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     1381 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     1441 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     1501 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     1561 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     1621 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     1681 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     1741 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     1801 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     1861 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     1921 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1981 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg gatccactag aaggccttag
     2041 tatgtatgta agttaataaa accctttttt ggagaatgta gatttaaaaa aacatatttt
     2101 ttttttattt tttactgcac tggacatcat tgaacttatc tgatcagttt taaatttact
     2161 tcgatccaag ggtatttgaa gtaccaggtt ctttcgatta cctctcactc aaaatgacat
     2221 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
     2281 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacctag cgtcaatgtc
     2341 cgccttcagt tgcactttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     2401 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     2461 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     2521 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     2581 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
     2641 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
     2701 aaaccgcaat cgcacaaaca aatagtgaca cgaaacagat tattctggta gctgtgctcg
     2761 ctatataaga caatttttaa gatcatatca tgatcaagac atctaaaggc attcattttc
     2821 gactacattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
     2881 acaaaaaata aataaaagta taaacctact tcgtaggata cttcgttttg ttcggggtta
     2941 gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg
     3001 agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct
     3061 cctgccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct
     3121 cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg
     3181 gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc
     3241 caggaaacat ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa
     3301 tcttaataaa gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc
     3361 gaacttttgg ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt
     3421 gcctttcgat cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc
     3481 caacctgtgg acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc
     3541 tttatcggct ccctaacggc cagggaacac ctgattttcc aggccatggt gcggatgcca
     3601 cgacatctga cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg
     3661 ctcagcaaat gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc
     3721 ggagaaagga agcgtctggc attcgcctcc gaggcactaa ccgatccgcc gcttctgatc
     3781 tgcgatgagc ccacctccgg actggactca tttaccgccc acagcgtcgt ccaggtgctg
     3841 aagaagctgt cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag
     3901 ctgtttgagc tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc
     3961 actcccagcg aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat
     4021 ctagcattac attacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta
     4081 caatccggcg gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc
     4141 ccgtgatcgg atcgccaaga tatgcgacaa ttttgctatt agcaaagtag cccgggatat
     4201 ggagcagttg ttggccacca aaaatttgga gaagccactg gagcagccgg agaatgggta
     4261 cacctacaag gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc
     4321 ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc
     4381 cagtggaaac aaatgatata acgcttacaa ttcttggaaa caaattcgct agattttagt
     4441 tagaattgcc tgattccaca cccttcttag tttttttcaa tgagatgtat agtttatagt
     4501 tttgcagaaa ataaataaat ttcatttaac tcgcgaacat gttgaagata tgaatattaa
     4561 tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt
     4621 tgggccaaca actcacgcaa gtgggcgtga tgaatatcaa cggagccatc ttcctcttcc
     4681 tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtctt gtttagaata
     4741 catttgcata ttaataattt actaactttc taatgaatcg attcgattta ggtgttcacc
     4801 tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac
     4861 tttctgggca aaacgattgc cgaattaccg ctttttctca cagtgccact ggtcttcacg
     4921 gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg
     4981 gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc
     5041 agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc
     5101 tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
     5161 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
     5221 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
     5281 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt
     5341 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
     5401 gagtagccga catatatccg aaataactgc ttgttttttt ttttaccatt attaccatcg
     5461 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
     5521 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
     5581 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
     5641 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
     5701 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
     5761 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
     5821 cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta
     5881 ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag
     5941 ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg
     6001 agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat
     6061 gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta
     6121 taataataag aatacattta atttagaaaa tgcttggatt tcactggaac tagaattaat
     6181 tcggctgctg ctctaaacga cgcatttcgt actccaaagt acgaattttt tccctcaagc
     6241 tcttattttc attaaacaat gaacaggacc taacgcacag tcacgttatt gtttacataa
     6301 atgatttttt ttactattca aacttactct gtttgtgtac tcccactggt atagccttct
     6361 tttatctttt ctggttcagg ctctatcact ttactaggta cggcatctgc gttgagtcgc
     6421 ctccttttaa atgtctgacc ttttgcaggt gcagccttcc actgcgaatc attaaagtgg
     6481 gtatcacaaa tttgggagtt ttcaccaagg ctgcacccaa ggctctgctc ccacaatttt
     6541 ctcttaatag cacacttcgg cacgtgaatt aattttactc cagtcacagc tttgcagcaa
     6601 aatttgcaat atttcatttt tttttattcc acgtaagggt taatgttttc aaaaaaaaat
     6661 tcgtccgcac acaacctttc ctctcaacaa gcaaacgtgc actgaattta agtgtatact
     6721 tcggtaagct tcggctatcg acgggaccac cttatgttat ttcatcatgg gccagaccca
     6781 cgtagtccag cggcagatcg gcggcggaga agttaagcgt ctccaggatg accttgcccg
     6841 aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc acgtccgccc
     6901 attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac gacaaccatt
     6961 tgaggtatac tggcaccgag cccgagttca agaagaaggc gtttttccat aggctccgcc
     7021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     7201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7441 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7561 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7681 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7741 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7801 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7861 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7921 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7981 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8041 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8101 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8161 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8221 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8281 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8341 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     8401 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8461 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8521 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8581 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8641 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8701 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8761 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8821 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8881 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8941 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9001 tgcaccatat gcggtgtgaa ataccgcacc gaatcgcgcg gaactaacga cagtcgctcc
     9061 aaggtcgtcg aacaaaaggt gaatgtgttg cggagagcgg gtgggagaca gcgaaagagc
     9121 aactacgaaa cgtggtgtgg tggaggtgaa ttatgaagag ggcgcgcgat ttgaaaagta
     9181 tgtatataaa aaatatatcc cggtgtttta tgtagcgata aacgagtttt tgatgtaagg
     9241 tatgcaggtg tgtaagtctt ttggttagaa gacaaatcca aagtctactt gtggggatgt
     9301 tcgaagggga aatacttgta ttctataggt catatcttgt ttttattggc acaaatataa
     9361 ttacattagc tttttgaggg ggcaataaac agtaaacacg atggtaataa tggtaaaaaa
     9421 aaaaacaagc agttatttcg gatatatgtc ggctactcct tgcgtcgggc ccgaagtctt
     9481 agagccagat atgcgagcac ccggaagctc acgatgagaa tggccagacc atgatgaaat
     9541 aacataaggt ggtcccgtcg gcaagagaca tccacttaac gtatgcttgc aataagtgcg
     9601 agtgaaagga atagtattct gagtgtcgta ttgagtctga gtgagacagc gatatgattg
     9661 ttgattaacc cttagcatgt ccgtggggtt tgaattaact cataatatta attagacgaa
     9721 attattttta aagttttatt tttaataatt tgcgagtacg caaagcttgg gctgcatctc
     9781 tccggatcca agcttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc
     9841 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     9901 cgagcggaga ctctagcgag cgccggagta taaatagagg cgcttcgtct acggagcgac
     9961 aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa
    10021 gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa
    10081 agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt
    10141 gaatacaaga agagaactct gaatagggaa ttgg
//
