LOCUS       sequence-109870-       11890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 11890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pCaSpeR4 mito roGFP2-Grx1
FEATURES             Location/Qualifiers
     source          1..11890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..83
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(108..110,111..821)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(150..171)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(411..430)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     758..779
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1393..1474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1430..1449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1484..1503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(1538..1739,1740..1770)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(2307..2326)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2426..2448
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2486..2504)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2572..2676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2677..2745,2746..3537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2895..2914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3708..4296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4197..4216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(4531..4561,4562..5116)
                     /label=P element 5' end
     gene            complement(5127..9262)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     9235..9253
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gcggccgcat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc tcggcccggc
       61 ggctcccagt gccgcgcgcc aagatccatt cgttgccgcc gggatccgtg agcaagggcg
      121 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      181 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      241 agttcatctc caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      301 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      361 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      421 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      481 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      541 acaactgcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
      601 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      661 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacctgct
      721 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      781 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gactagtggt ggttcaggtg
      841 gtggtggttc aggtggtggt ggttcaggtg gaggaggatc aggaggagga ggatcaggag
      901 gaggaggatc aggaggagaa ttcgctcaag agtttgtgaa ctgcaaaatc cagcctggga
      961 aggtggttgt gttcatcaag cccacctgcc cgtactgcag gagggcccaa gagatcctca
     1021 gtcaattgcc catcaaacaa gggcttctgg aatttgtcga tatcacagcc accaaccaca
     1081 ctaacgagat tcaagattat ttgcaacagc tcacgggagc aagaacggtg cctcgagtct
     1141 ttattggtaa agattgtata ggcggatgca gtgatctagt ctctttgcaa cagagtgggg
     1201 aactgctgac gcggctaaag cagattggag ctctgcagtc tagagtcgac ctcgaacgtt
     1261 aacgttaacg taacgttaac tcgagctaga tcataatcag ccataccaca tttgtaaagg
     1321 ttttaccgcg agaaaaaacc tcccacacct ccccctgaac ctgaaacata acttgtctgc
     1381 gttgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1441 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1501 atcaatgtat gtcgaggtcg acctgcagcc caagctttgc gtactcgcaa attattaaaa
     1561 ataaaacttt aaaaataatt tcgtctaatt aatattatga gttaattcaa accccacgga
     1621 catgctaagg gttaatcaac aatcatatcg ctgtctcact cagactcaat acgacactca
     1681 gaatactatt cctttcactc gcacttattg caagcatacg ttaagtggat gtctcttgcc
     1741 gacgggacca ccttatgtta tttcatcatg gtctggccat tctcatcgtg agcttccggg
     1801 tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc
     1861 gaaataactg cttgtttttt tttttaccat tattaccatc gtgtttactg tttattgccc
     1921 cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat
     1981 acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa
     2041 agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg
     2101 gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac
     2161 cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca
     2221 ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gtgcggtatt
     2281 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2341 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2401 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2461 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     2521 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2581 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2641 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2701 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     2761 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     2821 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     2881 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     2941 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3001 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3061 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3121 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3181 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3241 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3301 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3361 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3421 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3481 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3541 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3601 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3661 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3781 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3841 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4081 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4261 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccttcttctt gaactcgggc
     4321 tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag
     4381 ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg
     4441 aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc
     4501 gatctgccgc tggactacgt gggtctggcc catgatgaaa taacataagg tggtcccgtc
     4561 gatagccgaa gcttaccgaa gtatacactt aaattcagtg cacgtttgct tgttgagagg
     4621 aaaggttgtg tgcggacgaa tttttttttg aaaacattaa cccttacgtg gaataaaaaa
     4681 aaatgaaata ttgcaaattt tgctgcaaag ctgtgactgg agtaaaatta attcacgtgc
     4741 cgaagtgtgc tattaagaga aaattgtggg agcagagcct tgggtgcagc cttggtgaaa
     4801 actcccaaat ttgtgatacc cactttaaag attcgcagtg gaaggctgca cctgcaaaag
     4861 gtcagacatt taaaaggagg cgactcaacg cagatgccgt acctagtaaa gtgatagagc
     4921 ctgaaccaga aaagataaaa gaaggctata ccagtgggag tacacaaaca gagtaagttt
     4981 gaatagtaaa aaaaatcatt tatgtaaaca ataacgtgac tgtgcgttag gtcctgttca
     5041 ttgtttaatg aaaataagag cttgagggaa aaaattcgta ctttggagta cgaaatgcgt
     5101 cgtttagagc agcagccgaa ttaattctag ttccagtgaa atccaagcat tttctaaatt
     5161 aaatgtattc ttattattat agttgttatt tttgatatat ataaacaaca ctattatgcc
     5221 caccattttt ttgagatgca tctacacaag gaacaaacac tggatgtcac tttcagttca
     5281 aattgtaacg ctaatcactc cgaacaggtc acaaaaaatt accttaaaaa gtcataatat
     5341 taaattagaa taaatatagc tgtgagggaa atatatacaa atatattgga gcaaataaat
     5401 tgtacataca aatatttatt actaatttct attgagacga aatgaaccac tcggaaccat
     5461 ttgagcgaac cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg
     5521 tgaatgtgtt gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg
     5581 gtggaggtga attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc
     5641 ccggtgtttt atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct
     5701 tttggttaga agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt
     5761 attctatagg tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg
     5821 gggcaataaa cagtaaacac gatggtaata atggtaaaaa aaaaaacaag cagttatttc
     5881 ggatatatgt cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca
     5941 cccggaagct cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg
     6001 agaagttaag cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg
     6061 tgcagctaat ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg
     6121 cgtaacggaa ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt
     6181 tcaagaagaa gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg
     6241 ccatcgaggt cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg
     6301 ccaccagagt gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga
     6361 tcatcggata ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggtaattcgg
     6421 caatcgtttt gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca
     6481 taaaaactgg cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta
     6541 aattattaat atgcaaatgt attctaaaca agacttacat ttatcgtggc aaagacgttt
     6601 tgaaaggtca tgttggtcag gaagaggaag atggctccgt tgatattcat cacgcccact
     6661 tgcgtgagtt gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta
     6721 aaatgttact cgcatctcat taatattcat atcttcaaca tgttcgcgag ttaaatgaaa
     6781 tttatttatt ttctgcaaaa ctataaacta tacatctcat tgaaaaaaac taagaagggt
     6841 gtggaatcag gcaattctaa ctaaaatcta gcgaatttgt ttccaagaat tgtaagcgtt
     6901 atatcatttg tttccactgg aaccactcac cgttgtctga ataagtcgca cttttacgag
     6961 gagtggttcc ttgagcaccg acagccagga tcgccacagg accgcccgga actgcatgaa
     7021 ccaggtggcc ttgtaggtgt acccattctc cggctgctcc agtggcttct ccaaattttt
     7081 ggtggccaac aactgctcca tatcccgggc tactttgcta atagcaaaat tgtcgccata
     7141 tcttggcgat ccgatcacgg gactcgatct cccgtccggg cacaacggcc aacacctgta
     7201 cgtaaaagtc cgccggattg tagttggtag gacactgggc acccacgctg gataggagtt
     7261 gagatgtaat gtaatgctag atacccttaa taaacacatc gaactcacta ggaaaagaag
     7321 tcgacggctt cgctgggagt gcccaagaaa gctaccctgc cctcggccat cagaaggatc
     7381 ttgtcaaaga gctcaaacag ctcggaagac ggctgatgaa tggtcaggat gacggtcttg
     7441 cccttctgcg acagcttctt cagcacctgg acgacgctgt gggcggtaaa tgagtccagt
     7501 ccggaggtgg gctcatcgca gatcagaagc ggcggatcgg ttagtgcctc ggaggcgaat
     7561 gccagacgct tcctttctcc gccggacaga cctttcaccc tgccgggcac accgatgatc
     7621 gtgtgctgac atttgctgag cgaaagctcc tggatcacct gatccacgcg ggccactcgc
     7681 tgccgatagg tcagatgtcg tggcatccgc accatggcct ggaaaatcag gtgttccctg
     7741 gccgttaggg agccgataaa gaggtcatcc tgctggacat aggcgcacct ggcctgcatc
     7801 tccttggcgt ccacaggttg gccattgagc agtcgcatcc cggatggcga tacttggatg
     7861 ccctgcggcg atcgaaaggc aagggcattc agcagggtcg tctttccggc accggaactg
     7921 cccatcacgg ccaaaagttc gcccggatag gccacgccgc aaactgagtt tcaaattggt
     7981 aattggaccc tttattaaga tttcacacag atcagccgac tgcgaataga aactcaccgt
     8041 tcttgagcaa atgtttcctg ggcgccggta tgtgtcgctc gttgcagaat agtccgcgtg
     8101 tccggttgac cagctgccgc catccggagc ccggctgatt gaccgcccca aagatgtcca
     8161 tattgtgcca ggcataggtg aggttctcgg ctagttggcc gctccctgaa ccggagtcct
     8221 ccggcggact gggtggcagg agcgtgccgt agtttttggc ctgcccgaag ccctggttaa
     8281 tgcagctctg cgaagcgtcc gctgtcaccc tgcaatgata ggggatctca aatatcaact
     8341 acaagcgtta tgctcatcta accccgaaca aaacgaagta tcctacgaag taggtttata
     8401 cttttattta ttttttgtgc atctaggatc agcttaaaat atctggttgt tatatttttt
     8461 gtaaaaaaga atgtagtcga aaatgaatgc ctttagatgt cttgatcatg atatgatctt
     8521 aaaaattgtc ttatatagcg agcacagcta ccagaataat ctgtttcgtg tcactatttg
     8581 tttgtgcgat tgcggtttgg gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg
     8641 atgttgcaat cgcagttcct atagatcaag tgaacttaag atgtatgcac atgtactact
     8701 cacattgttc agatgctcgg cagatgggtg tttgctgcct ccgcgaatta atagctcctg
     8761 atcctcttgg cccattgccg ggatttttca cactttcccc tgcttaccca cccaaaacca
     8821 atcaccaccc caatcactca aaaaacaaac aaaaataaga agcgagagga gttttggcac
     8881 agcactttgt gtttaattga tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac
     8941 aaagtgcaac tgaaggcgga cattgacgct aggtaacgct acaaacggtg gcgaaagaga
     9001 tagcggacgc agcggcgaaa gagacggcga tatttctgtg gacagagaag gaggcaaaca
     9061 gcgctgactt tgagtggaat gtcattttga gtgagaggta atcgaaagaa cctggtactt
     9121 caaataccct tggatcgaag taaatttaaa actgatcaga taagttcaat gatatccagt
     9181 gcagtaaaaa ataaaaaaaa aatatgtttt tttaaatcta cattctccaa aaaagggttt
     9241 tattaactta catacatact agaattcgat atcaagcttg cacaggtcct gttcgataac
     9301 gtcgtactcg ggaatcacct ttaacttgtc cgctattggg tatttgtcct taaacacatt
     9361 cgtaatctct cccacaatca ttgccctggc ctctgcaatg taaaaagtat ttataaaata
     9421 tcgctggctc taaagaaaac atattaatca aagtattgag gctttatcaa tctaacattt
     9481 acaagtagtc agaccacaat ttttagactg cacttctgcg taagtaaatg aaatgaaatg
     9541 gtgacctact cttgggtagc cgtactgtta tatgctgacg agccttcacc tgggcctgct
     9601 ttttctcctg tttcgtctga tgcgtcggat gcggctggac tggctgattg ttgggattgg
     9661 gttttggatg ctgctccgaa aggtgggcca cagcttttgc gatggtggtg cccactatgc
     9721 ggttgctgca ggaactacag ctgaatgttc gctttattgt ctgcagctcc tggggctgga
     9781 cgaaggccac gccctcaccc ggtgcggcca tctcctgcgg caaaacgtat gtgggattcc
     9841 tgtccaagta gctctcgatg cccaccataa tgcgctccag caggttggcc aggtgcgggt
     9901 ctagctgcgg cggattcgtc gtctggttca ctgagtttag ggtctgcaga aagaagtgtg
     9961 cctccatggt aagcggattc atctgcacga tcagcgggct ttagtctaaa aattcgaatg
    10021 atacaaactc gtactcacgt aaagcagttt gtttggcatc atctcggcca tcgtcttatt
    10081 cttcttcgtg gcctttgtta ccggagcggc tatgttgggc gcggtggagc ccggaagctg
    10141 aaaggttaat cgaatgcgtt aacttttctg caactcgaaa gtttgccgcc tttgttcgac
    10201 tgccaataac tgttgattcg aaaattcgaa tcgaagcggt tgaatttcgt agggtggcca
    10261 actacaccaa agttcgccgg cggtgtatgc atgtgagtgt gagtgtgaca gtgggtgtac
    10321 gtgtgcttta agcgacacaa tcgcaccgcg atgtacatat ttttgtagaa atgtgtgtgc
    10381 atttttcgtc cggatcacgg ctccataaaa taaatcatat cacagggctc agggatttgt
    10441 gagtagaagt tcccgccact taaacgtcac ttgtgaaatc gccatcgacg gtcaactttt
    10501 actcacactg gtcttccgcg gacggaccgt ctcaaagtac tgcctttctg cgttggaaaa
    10561 catcgccttt ttcgtccaaa aggagtcccc aggttcgatc cgcatggcgt tgtgcgtgcg
    10621 tgcctttctt ttcaaatgat tacggctatt aacttggggg cgttaagttg gaaacacgta
    10681 aattgcagac tgcgattaga gtgaccatga gtaggagttc aaaatctcct gacatcattt
    10741 tcttaaaacc tgctttgttt tttacatttc tatttaatat aactcctatt tgaataaaaa
    10801 aacaaaacaa gtttagatgt taagatatta actacatcct ttgctccaaa gggagagggg
    10861 aagttatgga gttaattaat ttgctgttgg aaatcaatat ggagtcagaa atataatgat
    10921 ttactaaacc ttattgaatc ggtaacgatg cgaatttata ttaaaatagc ttttatgaaa
    10981 cattcaacaa aaatattatt aatgttggcc cactttagca accggttagg tctaccggtt
    11041 gggcaagcaa agattcacgc cctggttcga gtcccaacta gtcctgcaaa ataccgcagc
    11101 aagttttaga gagaccaagt gccattacct ctcccacttc agttatcggt tatgcggcgt
    11161 ttaagtcgac agcttgccgt ctctagctcc ggtgcctata taaagcagcc cgctttccac
    11221 atttcatatt cgttttacgt ttgtcaagcc tcatagccgg cagttcgaac gtatacgctc
    11281 tctgagtcag acctcgaaat cgtagctcta cacaattctg tgaattttcc ttgtcgcgtg
    11341 tgaaacactt ccaataaaaa ctcctgcagg tgagtacttt aaaaaaaaat ctagtgaaat
    11401 aatgctgaaa agaaatttgt gtgggcaaaa ttcaatgggc aaaaacgcga tgcggctttt
    11461 tctcaaaatg gcggccggcc tgcgtttttt cctcaaaagt gatgacgtca tgcctgtttt
    11521 ttttttttgt tcgcaatgag gaatggctct taaaatctac tagataaaaa aaatattcat
    11581 tatttctatg ctgctggaac gcttcattaa tcttaaaaat tctaaattcg gttaccatga
    11641 tacttcgacg cataactgta gattttggat agaattaaag agaaaatggc gagagagtaa
    11701 aattccggcg tcggcaaagt agagcaaaaa aatcagtata ccatttagct acctctctca
    11761 ctcgcacgca gtgccggctc aagttgggcg cggctctgca attatcgatt ttcttggggt
    11821 gtgtaactaa tcatccgttt tcccttcctc ctcatccaca gcgtgaaggt acccgcccgg
    11881 gatcagatcc
//
